A strain of bacteria in the Bacillus subtilis species complex was isolated from a building’s air vent in the Washington DC area, USA, and produced strong antifungal activity with in vitro assays. This strain, designated (HU Biol-II), showed pronounced inhibitory effects on mycelial growth of a wide spectrum of fungi. The objectives of this study were to use genome sequencing to confirm the taxonomy of HU Biol-II, evaluate its antifungal activity and implement genome mining and HPLC-MS/MS to characterize the bioactive secondary metabolites. The strain, as determined by multilocus sequence alignment analysis, was identified as a member of Bacillus subtilis subsp. inaquosorum clade. Core genome phylogeny showed that the isolate is most closely related to B. subtilis subsp. inaquosorum strain DE111, a commercially produced human probiotic. The investigation identified eight bioactive metabolite clusters in the genome. HPLC MS/MS was able to confirm the production of seven of the metabolites. This study is the first to report the production of two antifungal cyclic lipopeptides (bacillomycin F and fengycin) from a member of B. subtilis subsp. inaquosorum. The strain also produced the antibacterial aurantinin B, which confirms the biosynthetic cluster responsible for its production. Comparative genomics and metabolomics demonstrated the commercial probiotic strain DE111 produced the same metabolites, with the exception of aurantinin B. These findings are the first description of the secondary metabolites produced by a strain of B. subtilis subsp. inaquosorum.

从美国华盛顿特区某建筑物的通风口中分离出枯草芽孢杆菌种复合物中的一株细菌，并通过体外试验产生了很强的抗真菌活性。该菌株被命名为（HU Biol-II），对多种真菌的菌丝体生长均显示出明显的抑制作用。这项研究的目的是使用基因组测序来确认HU Biol-II的分类学，评估其抗真菌活性，并实施基因组挖掘和HPLC-MS / MS来表征生物活性次生代谢产物。通过多基因座序列比对分析确定的菌株被鉴定为枯草芽孢杆菌亚种的成员。绝种进化枝。核心基因组系统发育研究表明，该分离物与枯草芽孢杆菌亚种最密切相关。inaquosorum菌株DE111，一种商业生产的人益生菌。该研究确定了基因组中的八个生物活性代谢物簇。HPLC MS / MS能够确认其中7种代谢产物的产生。这项研究是第一个报告从枯草芽孢杆菌亚种成员中产生两种抗真菌环状脂肽（细菌霉素F和丰霉素）的研究。inaquosorum。该菌株还产生了抗菌素金黄色素B，这证实了负责其产生的生物合成簇。比较基因组学和代谢组学研究表明，商品商业益生菌DE111产生了相同的代谢产物，但aurantinin B除外。这些发现是对枯草芽孢杆菌亚种菌株产生的次生代谢产物的首次描述。inaquosorum。