We present a hybrid Monte Carlo simulation method with geometrical ray tracing (hMC-GRT) to model fluorescence excitation and detection in turbid media by optical imaging or spectroscopy systems employing a variety of optical components. hMC-GRT computational verification was achieved via reflectance and fluorescence simulations on epithelial tissue models in comparison with a standard Monte Carlo code. The mean difference between the two simulations was less than 5%. hMC-GRT experimental verification employed depth-sensitive steady-state fluorescence measurements using an aspherical lens on two-layered tissue phantoms. hMC-GRT predictions agreed well with experimental results, achieving less than 3.5% error for measurements at the phantom surface. Verification results demonstrate that the hMC-GRT simulation has the potential to become a useful computational toolbox for designing tissue fluorescence imaging and spectroscopy systems. In addition, the hMC-GRT approach enables a wide variety of applications for computational modeling of fluorescence in turbid media. The source codes are available at https://github.com/ubioptronics/hMC-GRT.

中文翻译：

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混合蒙特卡洛模拟与射线追踪，用于混浊介质中的荧光测量

我们提出了一种带有几何射线追踪（hMC-GRT）的混合蒙特卡罗模拟方法，以通过使用各种光学组件的光学成像或光谱系统对浊介质中的荧光激发和检测进行建模。与标准的蒙特卡洛代码相比，通过对上皮组织模型的反射和荧光模拟，实现了hMC-GRT的计算验证。两次模拟之间的平均差异小于5％。hMC-GRT实验验证在两层组织体模上使用非球面透镜对深度敏感的稳态荧光进行了测量。hMC-GRT预测与实验结果非常吻合，在幻像表面的测量误差小于3.5％。验证结果表明，hMC-GRT仿真具有成为设计组织荧光成像和光谱系统的有用计算工具的潜力。此外，hMC-GRT方法使浊度介质中的荧光计算模型能够广泛应用。可从https://github.com/ubioptronics/hMC-GRT获得源代码。