The objective of the present study was to understand mechanisms of inhibitory effect of l-carnosine on lipid oxidation using an oxidized myoglobin (metMb)-mediated washed cod muscle system. Experiments were conducted to compare l-carnosine with commercial antioxidants, tert-Butylhydroquinone (TBHQ) and α-tocopherol, and metal chelating reagent EDTA for inhibition of lipid oxidation, to compare l-carnosine with TBHQ for scavenging free radicals and to evaluate the effect of l-carnosine on structure of pro-oxidant metMb with spectral methods. Results showed that the inhibitory effect of l-carnosine on lipid oxidation was comparable with TBHQ and better than α-tocopherol. However, 1,1-diphenyl-2-picrylhydrazyl assay showed that TBHQ was much more effective in scavenging free radicals than l-carnosine. Comparing with 1.5% EDTA, 1.5% l-carnosine was much more effective against lipid oxidation. Incubation of l-carnosine with metMb resulted in spectral changes of metMb in wavelengths between 370 nm and 450 nm, Soret band shifted from 409 nm to 420 nm, and reduced molar ellepticity values. These results indicate that the high inhibitory effects of l-carnosine on meat lipid oxidation may result from not only its scavenging and chelating functions but also its effect on pro-oxidant metMb structure.

本研究的目的是了解的抑制作用机制升使用一种氧化的肌红蛋白（metMb）介导的洗涤鳕鱼肌肉系统-carnosine脂质氧化。进行实验来比较升-carnosine与商业抗氧化剂，叔丁基氢醌（TBHQ）和α生育酚，和金属螯合剂EDTA抑制脂质氧化，以比较升-carnosine与TBHQ用于清除自由基，以评价效果的升与谱方法助氧化剂metMb的结构-carnosine。结果表明，l的抑制作用-肌肽对脂质氧化的作用与TBHQ相当，并且优于α-生育酚。然而，1，1-二苯基-2-吡啶并肼基测定表明，TBHQ比1-肌肽更有效地清除自由基。与1.5％EDTA相比，1.5％的l-肌氨酸对脂质氧化的效果要好得多。将1-肌肽与metMb一起孵育会导致metMb在370 nm至450 nm之间的波长发生光谱变化，Soret谱带从409 nm移至420 nm，并降低了摩尔溶菌度。这些结果表明，高抑制作用升肉脂质氧化-carnosine可能导致不仅是它的清除和螯合功能，而且其对促氧化metMb结构的影响。