Abstract In this study, to recover proteins and minimize anti-nutritional factor (ANF) levels in soybean wastewater, a novel quick-shearing system was used to obtain homogeneous electrostatic complexes between soybean whey protein (SWP) and a high concentration (4%, w/v) of polysaccharide. During processing, protein recovery and trypsin inhibitory activity loss induced by complexing with sulfated and carboxylated polysaccharides were investigated as function of polysaccharide type, pH, protein to polysaccharide mass ratio, and the physical state (solid, liquid). The removal of 80% of ANF levels and recovery of 90% of proteins were achieved using solid sulfated polysaccharides. Large amounts of particle complexes were observed for sulfated polysaccharides as compared to carboxylated polysaccharides by confocal laser scanning microscopy. A 100-kDa polyethersulfone ultrafiltration membrane was effective in recovering proteins (80%) from ι-carrageenan/SWP complexes, and a small amount of loss of the polysaccharides was observed for each cycle.

中文翻译：

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新型快速剪切体系与高浓度多糖络合从大豆废水中回收蛋白质并去除抗营养因子

摘要 在本研究中，为了回收大豆废水中的蛋白质并最大限度地降低抗营养因子 (ANF) 水平，采用新型快速剪切系统获得大豆乳清蛋白 (SWP) 与高浓度 (4%, w/v) 的多糖。在加工过程中，研究了与硫酸化和羧化多糖复合引起的蛋白质回收和胰蛋白酶抑制活性损失，作为多糖类型、pH、蛋白质与多糖质量比和物理状态（固体、液体）的函数。使用固体硫酸化多糖可去除 80% 的 ANF 水平并回收 90% 的蛋白质。通过共聚焦激光扫描显微镜观察到与羧化多糖相比，硫酸化多糖有大量的颗粒复合物。