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Extensive Characterization of the Human Salivary Basic Proline-Rich Protein Family by Top-Down Mass Spectrometry
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-08-20 , DOI: 10.1021/acs.jproteome.8b00444 Alessandra Padiglia 1 , Roberto Orrù 1 , Mozhgan Boroumand 1 , Alessandra Olianas 1 , Barbara Manconi 1 , Maria Teresa Sanna 1 , Claudia Desiderio 2 , Federica Iavarone 3, 4 , Barbara Liori 1 , Irene Messana 2 , Massimo Castagnola 2, 3, 4 , Tiziana Cabras 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-08-20 , DOI: 10.1021/acs.jproteome.8b00444 Alessandra Padiglia 1 , Roberto Orrù 1 , Mozhgan Boroumand 1 , Alessandra Olianas 1 , Barbara Manconi 1 , Maria Teresa Sanna 1 , Claudia Desiderio 2 , Federica Iavarone 3, 4 , Barbara Liori 1 , Irene Messana 2 , Massimo Castagnola 2, 3, 4 , Tiziana Cabras 1
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Human basic proline-rich proteins and basic glycosylated proline-rich proteins, encoded by the polymorphic PRB1–4 genes and expressed only in parotid glands, are the most complex family of adult salivary proteins. The family includes 11 parent peptides/proteins and more than 6 parent glycosylated proteins, but a high number of proteoforms with rather similar structures derive from polymorphisms and post-translational modifications. 55 new components of the family were characterized by top-down liquid chromatography–mass spectrometry and tandem-mass platforms, bringing the total number of proteoforms to 109. The new components comprise the three variants P-H S1 → A, P-Ko P36 → S, and P-Ko A41 → S and several of their naturally occurring proteolytic fragments. The paper represents an updated reference for the peptides included in the heterogeneous family of proteins encoded by PRB1/PRB4. MS data are available via ProteomeXchange with the identifier PXD009813.
中文翻译：
自上而下质谱分析人类唾液富含脯氨酸的蛋白质家族的广泛表征
由多态性PRB1-4基因编码并且仅在腮腺中表达的人类碱性富含脯氨酸的蛋白质和碱性糖基化富含脯氨酸的蛋白质是成人唾液蛋白质中最复杂的家族。该家族包括11种亲本肽/蛋白质和6种以上亲本糖基化蛋白质，但是具有相当相似结构的大量蛋白形式来源于多态性和翻译后修饰。自上而下的液相色谱-质谱和串联质谱平台表征了该家族的55个新成分，从而使蛋白形式的总数达到109种。新成分包括PH S 1 →A，P-Ko P 36的三个变体。→S和P-Ko A 41→S及其一些天然存在的蛋白水解片段。本文代表了PRB1 / PRB4编码的蛋白质异质家族中包含的肽的最新参考。MS数据可通过ProteomeXchange获得，其标识符为PXD009813。
更新日期：2018-08-20
中文翻译：
自上而下质谱分析人类唾液富含脯氨酸的蛋白质家族的广泛表征
由多态性PRB1-4基因编码并且仅在腮腺中表达的人类碱性富含脯氨酸的蛋白质和碱性糖基化富含脯氨酸的蛋白质是成人唾液蛋白质中最复杂的家族。该家族包括11种亲本肽/蛋白质和6种以上亲本糖基化蛋白质，但是具有相当相似结构的大量蛋白形式来源于多态性和翻译后修饰。自上而下的液相色谱-质谱和串联质谱平台表征了该家族的55个新成分，从而使蛋白形式的总数达到109种。新成分包括PH S 1 →A，P-Ko P 36的三个变体。→S和P-Ko A 41→S及其一些天然存在的蛋白水解片段。本文代表了PRB1 / PRB4编码的蛋白质异质家族中包含的肽的最新参考。MS数据可通过ProteomeXchange获得，其标识符为PXD009813。
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