Mimicking the Chemistry of Natural Eumelanin Synthesis: The KE Sequence in Polypeptides and in Proteins Allows for a Specific Control of Nanosized Functional Polydopamine Formation,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Mimicking the Chemistry of Natural Eumelanin Synthesis: The KE Sequence in Polypeptides and in Proteins Allows for a Specific Control of Nanosized Functional Polydopamine Formation
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-07-30 00:00:00 , DOI: 10.1021/acs.biomac.8b00818
Camille Bergtold _{1,

2} , Daniel Hauser ₃ , Alain Chaumont ₄ , Salima El Yakhlifi ₂ , Mihaela Mateescu ₁ , Florent Meyer _{1,

2} , Marie-Hélène Metz-Boutigue _{1,

2} , Benoît Frisch ₅ , Pierre Schaaf _{1,

6} , Dris Ihiawakrim ₇ , Ovidiu Ersen ₇ , Christophe A. Monnier ₃ , Alke Petri-Fink ₃ , Barbara Rothen-Rutishauser ₃ , Vincent Ball _{1,

2}

Affiliation

The oxidation of dopamine and of other catecholamines leads to the formation of conformal films on the surface of all known materials and to the formation of a precipitate in solution. In some cases, it has been shown that the addition of additives in the dopamine solution, like certain surfactants or polymers, polyelectrolytes, and certain proteins, allows to get polydopamine nanoparticles of controlled size and the concomitant decrease, in an additive/dopamine dependent manner, in film formation on the surface of the reaction beaker. However, the mechanism behind this controlled oxidation and self-assembly of catecholamines is not known. In this article, it is shown that a specific diad of amino acids in proteins, namely KE, allows for specific control in the oxidation-self-assembly of dopamine to obtain [email protected] core–shell nanoparticles which are biocompatible. The interactions between dopamine and the adjacent KE amino acids potentially responsible for the size control of polydopamine aggregates was investigated by molecular dynamics simulations. The obtained core–shell nanoparticles display the biological activity of the protein used to control the self-assembly of PDA. The photon to heat conversion ability of PDA is conserved in the [email protected] particles.

中文翻译：

模仿天然Eumelanin合成的化学过程：多肽和蛋白质中的KE序列可对纳米级功能性多多巴胺的形成进行特定控制

多巴胺和其他儿茶酚胺的氧化导致在所有已知材料的表面上形成保形膜，并导致溶液中沉淀的形成。在某些情况下，已经表明，在多巴胺溶液中添加添加剂，例如某些表面活性剂或聚合物，聚电解质和某些蛋白质，可以使添加剂/多巴胺依赖的方式获得大小受控的聚多巴胺纳米颗粒，并随之减少，在反应烧杯表面成膜。然而，儿茶酚胺的这种受控氧化和自组装背后的机制尚不清楚。本文显示蛋白质中特定的氨基酸二元组，即KE，允许对多巴胺的氧化自组装进行特定控制，以获得具有生物相容性的[受电子邮件保护的]核壳纳米粒子。通过分子动力学模拟研究了多巴胺和潜在负责聚多巴胺聚集体尺寸控制的相邻KE氨基酸之间的相互作用。获得的核壳纳米粒子显示出用于控制PDA自组装的蛋白质的生物活性。PDA的光子至热转换能力在[电子邮件保护的]颗粒中得以保留。获得的核壳纳米粒子显示出用于控制PDA自组装的蛋白质的生物活性。PDA的光子至热转换能力在[电子邮件保护的]颗粒中得以保留。获得的核壳纳米粒子显示出用于控制PDA自组装的蛋白质的生物活性。PDA的光子至热转换能力在[电子邮件保护的]颗粒中得以保留。

更新日期：2018-07-30

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11