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Scoping Biocatalyst Performance Using Reaction Trajectory Analysis
Organic Process Research & Development ( IF 3.1 ) Pub Date : 2018-07-31 00:00:00 , DOI: 10.1021/acs.oprd.8b00119
Mathias Nordblad 1 , Mafalda Dias Gomes 1 , Murray P. Meissner 1 , Hemalata Ramesh 1 , John M. Woodley 1
Affiliation  

Process development for biocatalytic reactions is a complex task due to the required interaction of several different scientific disciplines. Additionally, there is a lack of standardized procedures for guiding development and for identifying the major process limitations in these systems. This work seeks to address this problem by providing a methodology based on a simple, systematic series of experiments. Application of the methodology helps identify the major bottleneck for process implementation, whether it be enzyme activity, enzyme stability, or substrate mass transfer. In addition, the underlying mechanism behind these limitations can also be inferred. The methodology is illustrated using a simulated reaction system and is also applied to three experimental case studies. This methodology provides a set of simple experiments that may be performed at an early stage of biocatalytic process development to guide effective improvement strategies, whether they be via protein engineering or reaction engineering. Ultimately, this should afford faster and more efficient implementation of biocatalysts in industrial processes.

中文翻译:

使用反应轨迹分析确定生物催化剂的性能

由于几种不同科学学科之间的相互作用,生物催化反应的工艺开发是一项复杂的任务。此外,缺乏用于指导开发和确定这些系统中主要过程限制的标准化程序。这项工作试图通过提供基于简单,系统的一系列实验的方法论来解决这个问题。该方法的应用有助于确定工艺实施的主要瓶颈,无论是酶活性,酶稳定性还是底物质量转移。此外,还可以推断出这些限制背后的潜在机制。使用模拟反应系统对方法进行了说明,并将其应用于三个实验案例研究。该方法学提供了一组简单的实验,可以在生物催化工艺开发的早期阶段进行,以指导有效的改进策略,无论是通过蛋白质工程还是通过反应工程。最终,这应该能够在工业过程中更快,更有效地实施生物催化剂。
更新日期:2018-07-31
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