High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

中文翻译：

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无伪影高密度定位显微镜分析。

用于定位显微镜的高密度分析方法提高了采集速度，但会产生伪影。我们证明了这些伪影可以通过将 Haar 小波核 (HAWK) 分析与标准单帧拟合相结合来消除。我们在合成、固定细胞和活细胞数据上测试了这种方法的性能，发现 HAWK 预处理产生了反映样本结构的重建，从而实现了对活体的高速、无伪影的超分辨率成像细胞。