Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors†,Materials Chemistry Frontiers

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Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors†
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2018-07-30 00:00:00 , DOI: 10.1039/c8qm00336j
Zhongxing Wang _{1,

2,

3,

4,

5} , Li Sun _{6,

7,

8} , Liqiang Liu _{1,

2,

3,

4,

5} , Hua Kuang _{1,

2,

3,

4,

5} , Liguang Xu _{1,

2,

3,

4,

5} , Chuanlai Xu _{1,

2,

3,

4,

5}

Affiliation

We developed an ultrasensitive gold nanoparticle-based multicomponent lateral-flow strip assay for the simultaneous detection of seventeen hormone drugs from three classes: nandrolone (NR) and its analogues, dexamethasone (DEX) and its analogues, and hexestrol (HES) and its analogues. The antibodies and coating antigens were selected for the assay and the multi-immunochromatographic strip (multi-ICS) exhibited a very low limit of detection (LOD). Quantitative results were obtained using a strip scan reader, giving LOD values of 0.06–4.85 ng mL⁻¹ for NR and its analogues, 0.005–2.85 ng mL⁻¹ for DEX and its analogues, and 0.03–2.14 ng mL⁻¹ for HES and its analogues. The recovery rates from milk samples were 85.8–93.3% for NR, 78.8–87.5% for DEX, and 89.4–99.4% for HES. This multi-ICS assay therefore provides a useful tool for on-site detection and rapid initial screening of hormone drugs in biological samples.

中文翻译：

使用纸质传感器同时超灵敏地检测17种化学物质†

我们开发了一种超灵敏的基于金纳米颗粒的多组分侧流条带测定法，用于同时检测三类中的17种激素药物：nandrolone（NR）及其类似物，地塞米松（DEX）及其类似物以及hexestrol（HES）及其类似物。选择抗体和包被抗原进行测定，并且多重免疫色谱带（multi-ICS）的检出限很低（LOD）。使用条扫描读取器得到的定量结果，给0.06-4.85纳克毫升LOD值^-1纳克mL的NR和其类似物，0.005-2.85 ^-1为DEX及其类似物，和0.03-2.14纳克毫升^-1用于HES及其类似物。NR的牛奶样品回收率为85.8–93.3％，DEX的回收率为78.8–87.5％，HES的回收率为89.4–99.4％。因此，这种多ICS分析方法为现场检测和快速初步筛选生物样品中的激素药物提供了有用的工具。

更新日期：2018-07-30

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