Silver nanoparticles (AgNPs) are being commercialized in a number of consumer products including food and cosmetics where there is a direct exposure of AgNPs to human body. An extensive toxicological evaluation is necessary to understand the mechanism for its safe use, since the toxicity effect varies greatly with the synthesis protocol followed. In this study, we report the detailed toxicological analysis of AgNPs fabricated by thermal co-reduction approach. Our study was analysed in human colon cancer cell line (HCT 116) and the IC₅₀ was calculated as 28.11 μg/ml. It was also observed that AgNP induces oxidative stress on HCT116 by increased levels of lipid peroxidation and reduced levels of glutathione. Mitochondrial membrane depolarization was also analysed and Western blot analysis confirms the increased level of Bcl and Caspase-3 which indicates the mitochondrial -mediated apoptosis. Additionally, flow cytometric analysis suggests cell cycle arrest in G2/M phase. Thus, our study can be a basis for further research to design safe AgNPs in various consumer products. Additionally, similar research can be conducted for different size and shape of AgNP or nano-silver can be engineered using different approaches.

银纳米颗粒（AgNPs）正在许多消费产品中商业化，包括食品和化妆品，这些产品直接将AgNPs暴露于人体。为了理解其安全使用机理，必须进行广泛的毒理学评估，因为毒性作用随所遵循的合成方案而有很大不同。在这项研究中，我们报告了通过热共还原法制备的AgNPs的详细毒理学分析。我们的研究在人结肠癌细胞系（HCT 116）和IC _{50中进行了分析}计算为28.11μg/ ml。还观察到，AgNP通过增加脂质过氧化水平和降低谷胱甘肽水平在HCT116上诱导氧化应激。线粒体膜去极化也进行了分析，蛋白质印迹分析证实了Bcl和Caspase-3的水平升高，表明线粒体介导的细胞凋亡。此外，流式细胞仪分析表明细胞周期停滞在G2 / M期。因此，我们的研究可以为进一步研究设计各种消费产品中的安全AgNPs奠定基础。此外，可以针对不同大小和形状的AgNP进行类似的研究，或者可以使用不同的方法设计纳米银。