The zebrafish, with its rapid external development, optical transparency, and the relative ease with which transgenic lines can be created, is rapidly becoming the model of choice for examining developmental processes via time-lapse microscopy. The recent proliferation of techniques for super-resolution imaging now allows for an unprecedented view of embryonic development at high spatial and temporal resolution in live tissues. This review examines both the theoretical basis and practical application of a number of established and emerging super-resolution microscopy techniques, focusing on their application in time-lapse imaging of live zebrafish embryos.

中文翻译：

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超越衍射极限的延时成像

斑马鱼具有快速的外部发育、光学透明度和相对容易的转基因系，正迅速成为通过延时显微镜检查发育过程的首选模型。最近超分辨率成像技术的激增现在允许在活组织中以高空间和时间分辨率对胚胎发育进行前所未有的观察。本综述审查了许多已建立和新兴的超分辨率显微镜技术的理论基础和实际应用，重点是它们在活斑马鱼胚胎的延时成像中的应用。