The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM. We were able to co-deposit the protein and silica at the electrified liquid–liquid interface via controlled proton transfer to the organic phase where it catalyzed tetraethoxysilane hydrolysis, followed by polycondensation to silica.

中文翻译：

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酸性磷酸酶在带电软结处的行为及其与二氧化硅的界面共沉积

在这项工作中研究了酸性磷酸酶在带电液-液界面上的行为。已经发现只有蛋白质的质子化形式可以经历界面吸附，这受水相pH的影响。使用离子转移伏安法，我们可以检测到浓度低至0.1μM的酸性磷酸酶。我们能够通过将质子转移到有机相中，从而催化四乙氧基硅烷水解，然后缩聚为二氧化硅，从而在电气化的液-液界面上共沉积蛋白质和二氧化硅。