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A cationic organoiridium(iii) complex-based AIEgen for selective light-up detection of rRNA and nucleolar staining†
Dalton Transactions ( IF 3.5 ) Pub Date : 2018-07-24 00:00:00 , DOI: 10.1039/c8dt02099j Sanjoy Kumar Sheet 1, 2, 3, 4, 5 , Bhaskar Sen 1, 2, 3, 4, 5 , Kripamoy Aguan 3, 4, 5, 6 , Snehadrinarayan Khatua 1, 2, 3, 4, 5
Dalton Transactions ( IF 3.5 ) Pub Date : 2018-07-24 00:00:00 , DOI: 10.1039/c8dt02099j Sanjoy Kumar Sheet 1, 2, 3, 4, 5 , Bhaskar Sen 1, 2, 3, 4, 5 , Kripamoy Aguan 3, 4, 5, 6 , Snehadrinarayan Khatua 1, 2, 3, 4, 5
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A green emissive cationic organoiridium(III) complex, 2[PF6], with a benzimidazole-substituted 1,2,3-triazole-pyridine (BiPT) ligand has been synthesized for target-specific cellular imaging and selective detection of ribosomal RNA (rRNA) over other competitive biomolecules in aqueous buffer solution at physiological pH. Complex 2 shows aggregation-induced emission enhancement (AIEE) properties and forms nano-aggregates in the presence of poor solvents. DFT and TD-DFT-based quantum mechanical calculations were performed to substantiate some photophysical features and to establish the intermolecular π–π interactions which detain the vibrational as well as rotational motions to form the aggregates, resulting in enhanced photoluminescence (PL). To corroborate the formation of nano-aggregates and to understand the morphology of the aggregated particles, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM) measurements were performed. 2[PF6] showed low cytotoxicity and good biocompatibility and was successfully employed in organelle-specific intracellular imaging. The in vivo and in vitro photoluminescence investigations affirmed that the probe stains cell nucleoli and selectively binds rRNA. It is assumed that the supramolecular π–π interactions between the benzimidazole of the BiPT ligand and the secondary structures of rRNA may facilitate aggregation and enable PL enhancement.
中文翻译：
基于 阳离子有机铱（iii）配合物的AIEgen，用于选择性点亮rRNA和核仁染色†
合成了带有苯并咪唑取代的1,2,3-三唑-吡啶（BiPT）配体的绿色发光阳离子有机铱（III）配合物2 [PF 6 ]，用于靶标特异性细胞成像和核糖体RNA的选择性检测（ rRNA）超过生理pH缓冲液中其他竞争性生物分子。复杂2显示出聚集体诱导的发射增强（AIEE）特性，并在不良溶剂存在下形成纳米聚集体。进行了基于DFT和TD-DFT的量子力学计算，以证实某些光物理特征并建立分子间π-π相互作用，从而阻止了振动和旋转运动，从而形成聚集体，从而增强了光致发光（PL）。为了证实纳米聚集体的形成并了解聚集颗粒的形态，进行了动态光散射（DLS），扫描电子显微镜（SEM），透射电子显微镜（TEM）和原子力显微镜（AFM）测量。2 [PF 6 ]具有低细胞毒性和良好的生物相容性，并已成功应用于细胞器特异性细胞内成像。在体内和体外的光致发光的研究确认，探针污渍细胞核仁和选择性结合的rRNA。假定BiPT配体的苯并咪唑与rRNA的二级结构之间的超分子π-π相互作用可能促进聚集并实现PL增强。
更新日期：2018-07-24
中文翻译：
基于 阳离子有机铱（iii）配合物的AIEgen，用于选择性点亮rRNA和核仁染色†
合成了带有苯并咪唑取代的1,2,3-三唑-吡啶（BiPT）配体的绿色发光阳离子有机铱（III）配合物2 [PF 6 ]，用于靶标特异性细胞成像和核糖体RNA的选择性检测（ rRNA）超过生理pH缓冲液中其他竞争性生物分子。复杂2显示出聚集体诱导的发射增强（AIEE）特性，并在不良溶剂存在下形成纳米聚集体。进行了基于DFT和TD-DFT的量子力学计算，以证实某些光物理特征并建立分子间π-π相互作用，从而阻止了振动和旋转运动，从而形成聚集体，从而增强了光致发光（PL）。为了证实纳米聚集体的形成并了解聚集颗粒的形态，进行了动态光散射（DLS），扫描电子显微镜（SEM），透射电子显微镜（TEM）和原子力显微镜（AFM）测量。2 [PF 6 ]具有低细胞毒性和良好的生物相容性，并已成功应用于细胞器特异性细胞内成像。在体内和体外的光致发光的研究确认，探针污渍细胞核仁和选择性结合的rRNA。假定BiPT配体的苯并咪唑与rRNA的二级结构之间的超分子π-π相互作用可能促进聚集并实现PL增强。
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