Zein, a prolamine obtained from maize, was employed to encapsulate a fish oil highly enriched with docosahexaenoic acid (DHA) by an innovative process termed electrospraying assisted by pressurized gas (EAPG). This technology combines high electric voltage with pneumatic spray to yield a high-throughput encapsulation process. Semi-spherical zein flowable capsules with mean sizes of 1.4 μm containing the DHA-enriched fish oil were produced by EAPG from inert ethanol solutions at room conditions, presenting a high encapsulation efficiency. The oxidative stability tests carried out in the zein microcapsules obtained by EAPG showed that the DHA-enriched fish oil was efficiently protected over storage time. Sensory tests were also performed on fortified reconstituted milk with the freshly prepared zein/DHA-enriched fish oil microcapsules, suggesting negligible oxidation effects after 45 days. The results described herein indicate that EAPG is a promising innovative high-throughput electrospraying-based methodology for the encapsulation of bioactives and, therefore, the resultant DHA-enriched fish oil containing microcapsules can be industrially applied for the formulation of fortified foods.

Industrial relevance

An innovative process, termed electrospraying assisted by pressurized gas (EAPG), is herein originally presented as a novel encapsulation methodology. This technology is based on the combination of high voltage and pneumatic spray, allowing the formation of microcapsules at room temperature conditions. Thus, EAPG shows a great deal of potential to encapsulate nutraceuticals and other bioactives that are sensitive to thermal degradation and/or oxidation. The resultant bioactive-containing capsules can be, thereafter, applied to develop novel fortified food products.

中文翻译：

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加压气体辅助电喷雾技术是一种创新的高通量方法，用于玉米醇溶蛋白醇溶蛋白中富含二十二碳六烯酸的鱼油的微囊化和稳定化

玉米醇溶蛋白，玉米蛋白，玉米醇溶蛋白，通过一种称为加压气体辅助电喷雾（EAPG）的创新方法，被用于封装高度富含二十二碳六烯酸（DHA）的鱼油。该技术将高电压与气动喷涂相结合，以实现高通量封装工艺。EAPG在室温下通过惰性乙醇溶液生产的平均大小为1.4μm的半球形玉米蛋白可流动胶囊，其中含有DHA富集的鱼油，具有很高的封装效率。在通过EAPG获得的玉米醇溶蛋白微胶囊中进行的氧化稳定性测试表明，富含DHA的鱼油可在储存时间内得到有效保护。还使用新鲜制备的富含玉米蛋白/ DHA的鱼油微胶囊对强化的重构牛奶进行了感官测试，表明45天后氧化作用可忽略不计。本文所述的结果表明，EAPG是用于生物活性物质封装的有前途的创新性高通量电子喷雾方法，因此，所得的富含DHA的鱼油微胶囊可在工业上用于强化食品的配制。

行业相关性

本文最初提出了一种创新的过程，称为加压气体辅助电喷（EAPG），是一种新颖的封装方法。该技术基于高压和气动喷涂的组合，可在室温条件下形成微囊。因此，EAPG显示出封装对热降解和/或氧化敏感的保健食品和其他生物活性物质的巨大潜力。然后，所得的含生物活性的胶囊可用于开发新型的强化食品。