A pilot-scale process combining extraction of Shiitake mushroom (Lentinula edodes) powder in water (98 °C, 1 h), cross-flow microfiltration and reverse osmosis (nanofiltration) was performed to obtain β-glucan-rich extracts. Suspensions (45–80 L) obtained after 3 extractions were clarified by microfiltration reducing their turbidity to <1 NTU. Membrane flux was completely recovered after filtration. One of the clarified extracts was concentrated (to 6–7 L) by reverse osmosis (Nanomax95) and the other two by nanofiltration (Nanomax50 and ALNF99-2517). Different physicochemical parameters (permeate flux, total soluble substances, total suspended particles and electrical conductivity) were monitored during filtration and the bioactive compounds present in the obtained fractions (β-glucans, total carbohydrates, chitins, eritadenine, lenthionine, ergosterol) were analyzed. The more adequate membrane for Shiitake extract concentration was Nanomax50 because it showed higher filtration flux and higher values of bioactive compounds in the obtained extract than the extracts obtained with the other two membranes.

Industrial relevance

This work describes a pilot-scale procedure for obtaining β-d-glucan-rich extracts from Lentinula edodes (Shiitake mushrooms). The extracts might be used in novel functional foods due to their high content in hypocholesterolemic compounds. The process combines extraction with boiling water, cross-flow membrane clarification and reverse osmosis/nanofiltration concentration of β-d-glucans. The procedure is scalable to industrial level.

中文翻译：

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通过萃取/微滤/反渗透（纳米过滤）工艺从香菇（香菇）中生产富含β-d-葡聚糖的提取物

香菇（香菇）提取的中试工艺）用水（98°C，1 h）中的粉末进行交叉流微滤和反渗透（纳米滤），以获得富含β-葡聚糖的提取物。3次萃取后得到的悬浮液（45–80 L）通过微滤得以澄清，将其浊度降低至<1 NTU。过滤后膜通量被完全回收。一种澄清的提取物通过反渗透（Nanomax95）浓缩（至6-7 L），另外两种通过纳滤（Nanomax50和ALNF99-2517）浓缩。在过滤过程中监测了不同的理化参数（渗透通量，总可溶性物质，总悬浮颗粒和电导率），并分析了所得馏分中的生物活性化合物（β-葡聚糖，总碳水化合物，几丁质，二十二碳五烯酸，硒代蛋氨酸，麦角固醇）。

行业相关性

这项工作描述了从香菇（香菇）中获得富含β- d-葡聚糖的提取物的中试规模的程序。由于提取物在降胆固醇的化合物中含量较高，因此可用于新型功能性食品。该方法将沸腾萃取，错流膜澄清和反渗透/纳滤浓缩的β- d-葡聚糖结合在一起。该程序可扩展到工业水平。