Extracellular electron transfer (EET) is believed to be a bottleneck in microbiologically influenced corrosion (MIC), also known as biocorrosion, by electro-active microbes. However, there is a lack of any direct evidence at the genetic level to confirm this so far in the literature. In this work, water-soluble electron transfer mediator molecule phenazine‑1‑carboxamide (PCN) produced by Pseudomonas aeruginosa was found to mediate the EET between P. aeruginosa and 2205 DSS (duplex stainless steel), thus regulating the corrosion rate. The phzH gene in P. aeruginosa encodes the enzyme which converts PCA (phenazine‑1‑carboxamide) to PCN. A P. aeruginosa mutant strain with phzH knockout (ΔphzH strain) and a strain with phzH restored (phzH-complemented mutant, phzH-comp.) were genetically engineered to confirm that phzH regulates EET-MIC of 2205 DSS by P. aeruginosa. Various electrochemical corrosion data in this work demonstrated that the corrosion rate decreased considerably after knocking out phzH and it largely recovered after phzH was restored. For the first time, it was demonstrated that a gene responsible for the production of an electron transfer mediator in P. aeruginosa regulated MIC, thus confirming at the genetic level that EET is a bottleneck in the MIC by P. aeruginosa.

人们认为，细胞外电子转移（EET）是电活性微生物在微生物影响的腐蚀（MIC）（也称为生物腐蚀）中的瓶颈。但是，到目前为止，在遗传水平上尚无任何直接证据可以证实这一点。在这项工作中，发现铜绿假单胞菌产生的水溶性电子转移介体分子吩嗪-1-羧酰胺（PCN）在铜绿假单胞菌和2205 DSS（双相不锈钢）之间介导了EET ，从而调节了腐蚀速率。铜绿假单胞菌中的phzH基因编码将PCA（吩嗪-1-羧酰胺）转化为PCN的酶。甲铜绿假单胞菌与突变菌株的phzH敲除（Δ的phzH菌株），并用一个应变的phzH恢复（的phzH -complemented突变体，的phzH -comp。）进行基因工程改造，以确认的phzH调节2205 DSS的EET-MIC由铜绿假单胞菌。这项工作中的各种电化学腐蚀数据表明，敲除phzH后腐蚀速率显着降低，并且在恢复phzH后很大程度上恢复了腐蚀速率。首次证明，负责在铜绿假单胞菌中产生电子传递介体的基因调节了MIC，从而在遗传水平上证实了EET是MIC的瓶颈。铜绿假单胞菌。