This paper reports a facile approach for the synthesis of alkaline protease nanoflowers–poly(vinyl alcohol) (PVA) composite hydrogel (NPCH) from alkaline protease–Cu₃(PO₄)₂·3H₂O nanoflowers and PVA hydrogel through freezing–thawing. During continuous catalytic application, the PVA hydrogel network protected alkaline protease–Cu₃(PO₄)₂·3H₂O nanoflowers from damage and helped maintain enzymatic activity at high levels. The enzyme that had been immobilized in nanoflowers and NPCH demonstrated 1027% and 605% higher activity than the free alkaline protease derived from Bacillus licheniformis. When used in cyclic catalysis, NPCH exhibited better reusability than nanoflowers and was easily separated from the product.

本文报道了一种从碱性蛋白酶-Cu ₃（PO ₄）₂ ·3H ₂ O纳米花和PVA水凝胶通过冻融合成碱性蛋白酶纳米花-聚乙烯醇（PVA）复合水凝胶（NPCH）的简便方法。。在连续催化应用过程中，PVA水凝胶网络可保护碱性蛋白酶– Cu ₃（PO ₄）₂ ·3H ₂ O纳米花免受损害，并有助于维持高水平的酶活性。固定在纳米花和NPCH中的酶显示出比地衣芽孢杆菌衍生的游离碱性蛋白酶高1027％和605％的活性。当用于循环催化时，NPCH比纳米花具有更好的可重复使用性，并且很容易从产物中分离出来。