当前位置: X-MOL 学术Photochem. Photobiol. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Pre-treatment of extracellular water soluble pigmented secondary metabolites of marine imperfect fungus protects HDF cells from UVB induced oxidative stress
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2018-07-23 00:00:00 , DOI: 10.1039/c8pp00221e
S. K. Shanuja 1, 2, 3, 4 , S. Iswarya 1, 2, 3, 4 , S. Rajasekaran 4, 5, 6 , M. G. Dinesh 4, 5, 6 , A. Gnanamani 1, 2, 3, 4
Affiliation  

The present study explores the UVB-induced oxidative stress protective efficacy of the pigmented fungal metabolite (identified as DHICA: 5,6-dihydroxyindole-2-carboxylic acid – a melanin precursor) using human dermal fibroblast (HDF) cells. DHICA is a water soluble pigment of the marine Aspergillus nidulans strain SG 28. Preliminary compatibility studies revealed 95% HDF cell viability with 600 μM concentration of DHICA. HDF cells were exposed to UVB irradiation with and without DHICA pre-treatment and the morphological, physiological and molecular level changes were observed accordingly. The results suggested that UVB exposure increases reactive oxygen species (ROS) generation and subsequent DNA damage in HDF cells, whereas DHICA pre-treatment appreciably reduces ROS generation and DNA damage. DHICA pre-treatment upregulates the antioxidant enzyme expressions and reduces the number of cells in the sub-Go/G1 phase. Gene expression analysis of TNF-α, IL-6, COX-2, NF-κB, Bax and Caspase 3 suggested that pre-treatment with DHICA downregulates the above-mentioned genes and simultaneously upregulates Bcl2 expression. In vivo experiments with BALB/c mice suggested that the topical application of DHICA protected mice skin from UVB-induced oxidative stress (which increases the epidermal thickness as evidenced in the skin sectioning). Thus, DHICA application protects the cells from UVB induced oxidative stress and may find applications in sunscreen cosmetic preparations.

中文翻译:

海洋不完全真菌的细胞外水溶性色素次级代谢产物的预处理可保护HDF细胞免受UVB诱导的氧化应激

本研究探讨了使用人类皮肤成纤维细胞(HDF)的有色真菌代谢物(鉴定为DHICA:5,6-二羟基吲哚-2-羧酸-黑色素前体)的UVB诱导的氧化应激保护作用。DHICA是海洋构巢曲霉的水溶性色素菌株SG28。初步的相容性研究表明,在600μM的DHICA浓度下,HDF细胞的活力为95%。HDF细胞在有和没有DHICA预处理的情况下都暴露于UVB照射下,因此观察到了形态,生理和分子水平的变化。结果表明,UVB暴露可增加HDF细胞中活性氧(ROS)的产生和随后的DNA损伤,而DHICA预处理可明显减少ROS的产生和DNA损伤。DHICA预处理可上调抗氧化酶的表达并减少sub-Go / G1期细胞的数量。TNF-α,IL-6,COX-2,NF-κB,Bax和Caspase 3的基因表达分析表明,用DHICA预处理可下调上述基因并同时上调Bcl2表达。体内BALB / c小鼠的实验表明,DHICA的局部应用可保护小鼠皮肤免受UVB诱导的氧化应激(如皮肤切片所示,可增加表皮厚度)。因此,DHICA的应用可以保护细胞免受UVB诱导的氧化应激的影响,并可以在防晒化妆品中找到应用。
更新日期:2018-07-23
down
wechat
bug