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Green synthesis of safe zero valent iron nanoparticles by Myrtus communis leaf extract as an effective agent for reducing excessive iron in iron-overloaded mice, a thalassemia model
RSC Advances ( IF 3.9 ) Pub Date : 2018-07-20 00:00:00 , DOI: 10.1039/c8ra04451a Shahram Eslami 1 , Mohammad Ali Ebrahimzadeh 1 , Pourya Biparva 2
RSC Advances ( IF 3.9 ) Pub Date : 2018-07-20 00:00:00 , DOI: 10.1039/c8ra04451a Shahram Eslami 1 , Mohammad Ali Ebrahimzadeh 1 , Pourya Biparva 2
Affiliation
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Green synthesis of Myrtus communis-Zero Valent Iron Nanoparticles (MC-ZVINs) was carried out in an alkaline environment. These nanoparticles were introduced for testing in vivo iron chelation in an iron-overload thalassemia model in mice. The MC-ZVINs were characterized by the use of imaging (electron microscopy) and spectroscopic (UV-vis and infrared absorption, dynamic light scattering and X-ray diffraction) methods. These methods demonstrated that some polyphenols are bound to the surfaces of the MC-ZVINs as a capping/stabilizing agent. The MC-ZVINs and defroxamine were injected 5 times a week over 4 weeks. The total iron and Fe3+ content of serum was determined by microwave plasma-atomic emission spectroscopy and an Fe3+ ion measuring kit respectively and liver enzyme levels were evaluated with an auto-analyzer. Morphological changes of the liver were examined by haematoxylin and eosin and Prussian blue staining. Additionally, the bioactivity of the iron nanoparticles was checked by DPPH and nitric oxide radical scavenging activity, reducing power and iron (Fe2+) chelating ability. Iron-overloaded mice treated with the MC-ZVINs, indicated a drastic reduction in serum iron content when compared with the control groups and those treated with defroxamine. MC-ZVINs exhibit satisfactory potency to chelate excessive iron in mice. The data collected in this study will be helpful for selecting the most appropriate operational conditions for synthesizing green ZVINs in biomedical applications.
中文翻译：
桃金娘叶提取物绿色合成安全的零价铁纳米颗粒作为降低铁过载小鼠地中海贫血模型过量铁的有效药剂
桃金娘-零价铁纳米粒子（MC- ZVINs ）的绿色合成在碱性环境中进行。引入这些纳米颗粒用于在小鼠的铁过载地中海贫血模型中测试体内铁螯合。通过使用成像（电子显微镜）和光谱（UV-vis 和红外吸收、动态光散射和 X 射线衍射）方法对 MC-ZVIN 进行表征。这些方法表明，一些多酚作为封端/稳定剂结合到 MC-ZVIN 的表面。MC-ZVINs 和 defroxamine 在 4 周内每周注射 5 次。采用微波等离子体原子发射光谱法测定血清中总铁和 Fe 3+含量，采用 Fe 3+离子测量试剂盒分别用自动分析仪评估肝酶水平。通过苏木精和伊红和普鲁士蓝染色检查肝脏的形态变化。此外，铁纳米粒子的生物活性通过DPPH和一氧化氮自由基清除活性、还原能力和铁(Fe 2+ )螯合能力来检查。用 MC-ZVINs 治疗的铁超负荷小鼠与对照组和用去甲氧胺治疗的小鼠相比，血清铁含量急剧下降。MC-ZVIN 表现出令人满意的螯合小鼠体内过量铁的效力。本研究收集的数据将有助于在生物医学应用中为合成绿色 ZVIN 选择最合适的操作条件。
更新日期：2018-07-20
中文翻译：
桃金娘叶提取物绿色合成安全的零价铁纳米颗粒作为降低铁过载小鼠地中海贫血模型过量铁的有效药剂
桃金娘-零价铁纳米粒子（MC- ZVINs ）的绿色合成在碱性环境中进行。引入这些纳米颗粒用于在小鼠的铁过载地中海贫血模型中测试体内铁螯合。通过使用成像（电子显微镜）和光谱（UV-vis 和红外吸收、动态光散射和 X 射线衍射）方法对 MC-ZVIN 进行表征。这些方法表明，一些多酚作为封端/稳定剂结合到 MC-ZVIN 的表面。MC-ZVINs 和 defroxamine 在 4 周内每周注射 5 次。采用微波等离子体原子发射光谱法测定血清中总铁和 Fe 3+含量，采用 Fe 3+离子测量试剂盒分别用自动分析仪评估肝酶水平。通过苏木精和伊红和普鲁士蓝染色检查肝脏的形态变化。此外，铁纳米粒子的生物活性通过DPPH和一氧化氮自由基清除活性、还原能力和铁(Fe 2+ )螯合能力来检查。用 MC-ZVINs 治疗的铁超负荷小鼠与对照组和用去甲氧胺治疗的小鼠相比，血清铁含量急剧下降。MC-ZVIN 表现出令人满意的螯合小鼠体内过量铁的效力。本研究收集的数据将有助于在生物医学应用中为合成绿色 ZVIN 选择最合适的操作条件。
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