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Insights into the carbonic anhydrases and autotrophic carbon dioxide fixation pathways of high CO2 tolerant Rhodovulum viride JA756
Microbiological Research ( IF 6.7 ) Pub Date : 2018-07-20 , DOI: 10.1016/j.micres.2018.07.006
Lakshmi Venkata Naga Satya Khandavalli , Tushar Lodha , Maaged Abdullah , Lalitha Guruprasad , Sasikala Chintalapati , Venkata Ramana Chintalapati

Biofixation of CO2 is being extensively investigated to solve the global warming problem. Purple non-sulfur bacteria are fast growers that consume CO2 and produce beneficial biomass. Better the growth at higher CO2 levels, more efficient are the strains for biofixation. Nine among fifty strains that were analyzed at elevated CO2 levels responded with better growth. Considering its enhanced growth at high CO2 and metabolic versatility, Rhodovulum viride strain JA756 was chosen to make further studies. Strain JA756 tolerates up to 50% (v/v) CO2 with its optimum between 20–40% (v/v), yielding a biomass of 3.4 g. L−1. The pattern of specific enzyme activity of carbonic anhydrase corresponded well with that of its growth. To gain insights into the genomic composition and genes related to carbonic anhydrases and CO2 fixation, draft genome sequencing of JA756 was carried out which revealed the presence of two non-homologous genes encoding for β and γ carbonic anhydrases, both of which are assumed to be implicated in maintaining intracellular inorganic carbon concentration at equilibrium. Most of the genes involved in the Calvin pathway, reductive tricarboxylic acid pathway, 3-hydroxypropionate bicycle and C4 pathways were found in the draft genome. While the experimental determinations of active roles of two of these pathways are still underway, the expression of key genes of Calvin and C4 pathway suggest their functional role in the organism. Owing to its metabolic versatility, JA756 can be advantageous for biological CO2 assimilation facilities located by the coastline, inland and also at wide ranges of CO2 concentrations.



中文翻译:

深入了解高耐受CO 2的拟南芥(Rhodovulum viride) JA756的碳酸酐酶和自养二氧化碳固定途径

为了解决全球变暖问题,正在对CO 2的生物固定进行广泛研究。紫色非硫细菌是速生植物,消耗CO 2并产生有益的生物量。在较高的CO 2水平下生长更好,生物固定菌株更有效。在升高的CO 2水平下分析的五十个菌株中,有九个响应较好的生长。考虑到其在高CO 2下的生长增强和新陈代谢的多功能性,选择了杜鹃红景天菌株JA756进行进一步研究。JA756菌株最多可耐受50%(v / v)的CO 2,其最佳浓度为20-40%(v / v),可产生3.4 g的生物量。L -1。碳酸酐酶的特定酶活性模式与其生长模式非常吻合。为了深入了解与碳酸酐酶和CO 2固定相关的基因组组成和基因,对JA756进行了基因组测序草案,揭示了存在两个编码β和γ碳酸酐酶的非同源基因,这两个基因均假定为与维持细胞内无机碳浓度平衡有关。在基因组草图中发现了大多数与Calvin途径,还原性三羧酸途径,3-羟基丙酸酯自行车和C 4途径有关的基因。尽管仍在实验确定其中两种途径的活跃作用,但Calvin和C关键基因的表达仍在进行中。4个途径提示它们在生物体中的功能作用。由于其代谢的多功能性,JA756对于位于海岸线,内陆以及广泛的CO 2浓度范围内的生物CO 2同化设施可能是有利的。

更新日期:2018-07-20
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