MtLPMO9 and FoLPMO9 are two lytic polysaccharide monooxygenases (LPMOs), from the filamentous fungi Thermothelomyces thermophila and Fusarium oxysporum, respectively. In the present study an attempt has been made to achieve direct electron transfer between these enzymes and a glassy carbon electrode by immobilization in Nafion polyelectrolyte. The method used to ascertain the feasibility of direct electron transfer was large amplitude Fourier transform alternating current voltammetry (FTacV) and the formal potentials of these enzymes were determined at different temperatures. The findings of this paper indicate that LPMOs can be studied by direct electron transfer, which could be exploited in the near future for their biochemical characterization.

Mt LPMO9和Fo LPMO9是两种溶解性多糖单加氧酶（LPMO），来自丝状真菌嗜热栖热菌和尖孢镰刀菌， 分别。在本研究中，已经尝试通过固定在Nafion聚电解质中来实现这些酶和玻璃碳电极之间的直接电子转移。用于确定直接电子转移可行性的方法是大振幅傅立叶变换交流伏安法（FTacV），并在不同温度下确定这些酶的形式势。本文的发现表明，可以通过直接电子转移研究LPMO，这将在不久的将来用于其生化表征。