The medial prefrontal cortex (mPFC) is an important brain region, that controls a variety of behavioral and functional outputs. As an important step in characterizing mPFC functionality, in this paper we focus on chemically defining serotonin transmission in this area. We apply cutting-edge analytical methods, fast-scan cyclic voltammetry (FSCV) and fast-scan controlled adsorption cyclic voltammetry (FSCAV), pioneered in our laboratory, for the first real-time in vivo analysis of serotonin in the mPFC. In prior in vivo work in the substantia nigra, pars reticulata, we found that our sub-second measurements of a single evoked serotonin release were subject to two clearance mechanisms. These mechanisms were readily modeled via Uptake 1, mediated by the serotonin transporters (SERTs), and Uptake 2, mediated by monoamine transporters (dopamine transporters (DATs), norepinephrine transporters (NETs), and organic cation transporters (OCTs)). Here in the mPFC, for the first time to our knowledge, we observe two release events in response to a single stimulation of the medial forebrain bundle (MFB). Of particular note is that each response is tied to a discrete reuptake profile comprising both Uptake 1 and 2. We hypothesize that two distinct populations of serotonin axons traverse the MFB and terminate in different domains with specific reuptake profiles. We test and confirm this hypothesis using a multifaceted pharmacological, histological and mathematical approach. We thus present evidence for a highly elaborate biochemical organization that regulates serotonin chemistry in the mPFC. This knowledge provides a solid foundation on which to base future studies of the involvement of the mPFC in brain function and behavior.

中文翻译：

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伏安法证明了小鼠内侧前额叶皮层中与特定再摄取域相关的离散5-羟色胺回路。

内侧前额叶皮层（mPFC）是重要的大脑区域，控制着各种行为和功能输出。作为表征mPFC功能的重要步骤，在本文中，我们着重于化学定义该区域中血清素的传递。我们将最先进的分析方法，快速扫描循环伏安法（FSCV）和快速扫描受控吸附循环伏安法（FSCAV）应用于我们的实验室，首次对mPFC中的血清素进行了实时体内分析。在先前在黑质黑质网中的体内工作中，我们发现对单个诱发的5-羟色胺释放的亚秒测量受两个清除机制的影响。这些机制很容易通过血清素转运蛋白（SERT）介导的摄取1和摄取2来建模。由单胺转运蛋白（多巴胺转运蛋白（DAT），去甲肾上腺素转运蛋白（NETs）和有机阳离子转运蛋白（OCTs））介导。在mPFC中，据我们所知，这是我们第一次观察到对内侧前脑束（MFB）的单次刺激而产生的两个释放事件。特别值得注意的是，每个响应都与包含摄取1和2的离散重摄取谱相关。我们假设，血清素轴突的两个不同群体遍历MFB，并终止于具有特定重摄取谱的不同域中。我们使用多方面的药理学，组织学和数学方法测试并证实了这一假设。因此，我们为高度精细的生化组织提供了证据，该组织可调节mPFC中的血清素化学。