Chlordecone and fipronil are used as an insecticide and have been widely detected in the aquatic environments. However, their toxicity is still poorly investigated in aquatic invertebrates. In this study, we examined effects of chlordecone and fipronil on population growth and transcriptional regulation of the entire cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and the marine rotifer B. plicatilis. In B. calyciflorus, a 24 h-no observed effect concentration (NOEC-24 h) and a 24 h-median lethal concentration (LC50-24 h) of chlordecone were determined as 100 μg/L and 193.8 μg/L, respectively, while NOEC-24 h and LC50-24 h of fipronil were determined as 1000 μg/L and 2033.0 μg/L, respectively. In B. plicatilis, NOEC-24 h and LC50-24 h of chlordecone were 100 μg/L and 291.0 μg/L, respectively, while NOEC-24 h and LC50-24 h of fipronil were determined as 1000 μg/L and 5735.0 μg/L, respectively. Moreover, retardation in the population growth were observed in response to chlordecone and fipronil in both rotifer species, suggesting that chlordecone and fipronil have a potential adverse effects on life cycle parameters of two rotifer species. Additionally, modulation in the expressions of the entire CYP genes were demonstrated in response to chlordecone and fipronil at 24 h period. These results provide the better understanding on how chlordecone and fipronil can affect in population growth of two rotifers and CYP gene expressions in chlordecone- and fipronil-exposed rotifers.

十氯酮和氟虫腈被用作杀虫剂，并已在水生环境中被广泛检测到。但是，它们的毒性在水生无脊椎动物中仍未得到很好的研究。在这项研究中，我们检查了十氯酮和氟虫腈对淡水轮虫Brachionus calyciflorus和海洋轮虫B. plicatilis中种群生长和整个细胞色素P450（CYP）基因转录调控的影响。在轮虫，24 H-无作用浓度（NOEC-24 h）和一个24小时的中值致死浓度（LC50-24 1H）十氯酮的测定为100微克/ L和193.8微克/升，氟虫腈的NOEC-24 h和LC50-24 h分别测定为1000μg/ L和2033.0μg/ L。在B. plicatilis，十氯酮的NOEC-24 h和LC50-24 h分别为100μg/ L和291.0μg/ L，而氟虫腈的NOEC-24 h和LC50-24 h分别为1000μg/ L和5735.0μg / L。此外，在两种轮虫中都观察到了响应十氯酮和氟虫腈的种群增长减慢，这表明十氯酮和氟虫腈对两种轮虫的生命周期参数具有潜在的不利影响。另外，在24小时内，响应于十氯酮和氟虫腈，证明了整个CYP基因表达的调节。这些结果更好地了解了十氯酮和氟虫腈如何影响两个轮虫和CYP的种群增长 十氯酮和氟虫腈暴露的轮虫中的基因表达。