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Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-07-31 , DOI: 10.1021/acs.iecr.8b02195 Jun Cui 1 , Lin Li 1, 2, 3 , Lingmei Kou 1 , Hui Rong 4 , Bing Li 1, 3 , Xia Zhang 1, 3
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-07-31 , DOI: 10.1021/acs.iecr.8b02195 Jun Cui 1 , Lin Li 1, 2, 3 , Lingmei Kou 1 , Hui Rong 4 , Bing Li 1, 3 , Xia Zhang 1, 3
Affiliation
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A novel, magnetic three-phase fluidized bed reactor (MTFBR) was designed with magnetic immobilized cellulase (MIC) as the biocatalyst for preparing chitooligosaccharides from chitosan. The MIC showed higher enzyme activity in a pulse magnetic field (PMF) than that in a steady magnetic field (SMF) or alternating magnetic field (AMF) under certain operating parameters. The application of a magnetic field increased the maximum reaction rate (Vmax) and Michaelis constant (Km), and the reaction rates of the MIC-catalyzed reaction in PMF and SMF at high concentration of chitosan solution (Cs, 5 < Cs < 20 mg/mL) increased as the intensity of the magnetic field increased. The concentrations of chitosan pentamer and hexamer produced were higher in the PMF than in the SMF and AMF, and the application of a magnetic field greatly reduced (by as much as 37.5%) the biocatalytic reaction time required to reach the maximum concentration of the desired chitooligosaccharides. These results suggest that the customized MTFBR supplemented with MIC is a reusable and effective piece of biocatalytic equipment, indicating that it has potential applications for large industrial-scale chitooligosaccharide syntheses.
中文翻译：
三种磁场在磁性三相流化床反应器中固定化纤维素酶活性的比较
设计了一种新型的磁性三相流化床反应器（MTFBR），以磁性固定纤维素酶（MIC）为生物催化剂，由壳聚糖制备壳寡糖。在某些操作参数下，MIC在脉冲磁场（PMF）中的酶活性高于稳定磁场（SMF）或交变磁场（AMF）的酶活性。磁场的施加提高了壳聚糖溶液在高浓度（C s，5 < C）下最大反应速率（V max）和米氏常数（K m），以及MIC催化的PMF和SMF中的反应速率。s<20 mg / mL）随着磁场强度的增加而增加。PMF中产生的壳聚糖五聚体和六聚体的浓度高于SMF和AMF中的浓度，并且磁场的施加极大地降低了（达到37.5％的）生物催化反应时间，以达到所需的最大浓度。壳寡糖。这些结果表明，定制的添加了MIC的MTFBR是一种可重复使用且有效的生物催化设备，表明其在大规模工业规模的壳寡糖合成中具有潜在的应用。
更新日期：2018-08-01
中文翻译：
三种磁场在磁性三相流化床反应器中固定化纤维素酶活性的比较
设计了一种新型的磁性三相流化床反应器（MTFBR），以磁性固定纤维素酶（MIC）为生物催化剂，由壳聚糖制备壳寡糖。在某些操作参数下，MIC在脉冲磁场（PMF）中的酶活性高于稳定磁场（SMF）或交变磁场（AMF）的酶活性。磁场的施加提高了壳聚糖溶液在高浓度（C s，5 < C）下最大反应速率（V max）和米氏常数（K m），以及MIC催化的PMF和SMF中的反应速率。s<20 mg / mL）随着磁场强度的增加而增加。PMF中产生的壳聚糖五聚体和六聚体的浓度高于SMF和AMF中的浓度，并且磁场的施加极大地降低了（达到37.5％的）生物催化反应时间，以达到所需的最大浓度。壳寡糖。这些结果表明，定制的添加了MIC的MTFBR是一种可重复使用且有效的生物催化设备，表明其在大规模工业规模的壳寡糖合成中具有潜在的应用。
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