Abstract Extracts rich in monomeric anthocyanins (MAC) and total phenolic compounds (TPC) were obtained from grape marc by Pressurized Liquid Extraction (PLE). PLE was performed using ethanol and water mixtures (acidified or not) (50% w/w), pure ethanol and acidified water at temperatures from 40 to 100 °C. The best PLE conditions for MAC extraction (ethanol-water pH 2.0 [50% w/w]) resulted in 10.21 mg of malvidin-3-O-glucoside/g of dried grape marc (dr). Fifteen anthocyanins were identified and quantified in PLE extracts by UHPLC-UV-Vis. PLE with ethanol-water (50% w/w) as solvent at 100 °C achieved the highest TPC content (65.68 mg GAE/g dr) and antioxidant capacity by ORAC (772.11 μmol TE/g dr) and FRAP (1452 mg TE/g dr) among the evaluated conditions. Based on the results for extraction of monomeric anthocyanins and phenolics compounds, a sequential PLE process was performed and proved to be viable for the recovery of two different extract fractions.

中文翻译：

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从葡萄渣中加压液体提取生物活性化合物

摘要 采用加压液体萃取法（PLE）从葡萄渣中提取了富含单体花青素（MAC）和总酚类化合物（TPC）的提取物。PLE 使用乙醇和水的混合物（酸化或未酸化）（50% w/w）、纯乙醇和酸化水在 40 至 100 °C 的温度下进行。MAC 提取的最佳 PLE 条件（乙醇 - 水 pH 值 2.0 [50% w/w]）导致 10.21 mg malvidin-3-O-葡萄糖苷/g 干葡萄渣 (dr)。通过 UHPLC-UV-Vis 在 PLE 提取物中鉴定并定量了 15 种花青素。PLE 以乙醇-水 (50% w/w) 作为溶剂在 100 °C 下达到最高的 TPC 含量 (65.68 mg GAE/g dr) 和 ORAC (772.11 μmol TE/g dr) 和 FRAP (1452 mg TE) 的抗氧化能力/g dr) 在评估条件中。基于单体花青素和酚类化合物的提取结果，