Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported. The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings. Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8–10 colony forming units (CFU). There was a 3–4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C.

存在几种用于研究慢性伤口感染的模型，但是很少有模型结合了所有必要的要素以实现高通量，可重现的生物膜培养以及应用局部抗菌治疗的可能性。此外，很少有人考虑在气液界面上提供营养和生物膜生长的适当方法。在此手稿中，报道了一种用于研究伤口生物膜的新型生物膜流动装置。该设备是3D打印的，易于操作，可用于研究单一和混合物种的生物膜，以及抗菌敷料的功效。金黄色葡萄球菌或铜绿假单胞菌的单一物种生物膜在72小时内可重复培养，得到一致的对数计数，即8-10个菌落形成单位（CFU）。当将抗微生物敷料应用于培养48 h的生物膜，并原位放置24 h时，可恢复细菌减少3-4 log 。分别以1：1比例接种的金黄色葡萄球菌和铜绿假单胞菌的两种生物膜也可分别在20°C和37°C下培养。特别值得注意的是，只有在37°C时，人群中的革兰氏阴性变化才发生。