Selenium nanoparticles paved its way into the field of medicine because of its lowered toxicity and excellent biological properties. Interaction between these nanoparticles and serum proteins leads to the formation of protein corona (hard and soft) on the nanoparticle surface modulating its biological behaviour and fate. In the present study, we have emphasized on the biocorona formation of most abundant serum proteins i.e. human serum albumin, immunoglobulin-G, and transferrin on functionalized selenium nanoparticles (CTAB, SDS, and brij-58). Changes in protein adsorption pattern, mean hydrodynamic size, and zeta potential of differently functionalized selenium nanoparticles, following protein coronation were analysed for various incubation periods. Quantification of hard corona and soft corona for individual protein and for protein mixture was also done. The biological impact of protein coronation on selenium nanoparticles was evaluated by the cellular uptake and cell viability studies. Finally, modulation in radical scavenging property of selenium nanoparticles before and after protein corona formation was studied through DPPH assay.

硒纳米粒子具有较低的毒性和出色的生物学特性，从而进入了医学领域。这些纳米粒子与血清蛋白之间的相互作用导致纳米粒子表面蛋白质电晕（硬和软）的形成，从而调节其生物学行为和命运。在本研究中，我们着重介绍了功能丰富的硒纳米颗粒（CTAB，SDS和brij-58）上最丰富的血清蛋白（即人血清白蛋白，免疫球蛋白G和转铁蛋白）的生物电晕形成。在不同的潜伏期中，分析了蛋白质加冕后蛋白质功能不同的硒纳米颗粒的蛋白质吸附模式，平均流体动力学大小和ζ电位的变化。还对单个蛋白质和蛋白质混合物的硬质电晕和软质电晕进行了定量。通过细胞摄取和细胞活力研究评估了蛋白质加冕对硒纳米颗粒的生物学影响。最后，通过DPPH分析研究了蛋白质电晕形成前后硒纳米颗粒自由基清除性能的调控。