We report herein a new dicyanoisophorone-based colorimetric and near-infrared (NIR) fluorescent probe for rapid detection of thiophenols. This probe possesses a remarkable large Stokes shift (208 nm) and works in almost pure aqueous solution under mild conditions. More importantly, it displays a rapid, highly selective and sensitive dual colorimetric and NIR fluorescent turn-on signal responses for thiophenols and allows the detection of thiophenols in the presence of analytes including amino acids, biothiols and aliphatic thiols. Moreover, the fluorescence of this probe at 655 nm displays a good linear relationship with the thiophenol concentration ranging from 0 to 20 μM, and the detection limit is as low as 12 nM. Furthermore, the practicability of this new probe has been successfully proved by detection of thiophenol in real water samples and bioimaging of thiophenol in living cells.

我们在此报告了一种新的基于双氰基异佛尔酮的比色和近红外（NIR）荧光探针，用于快速检测苯硫酚。该探针具有显着的大斯托克斯位移（208 nm），可在温和条件下在几乎纯净的水溶液中工作。更重要的是，它对硫酚显示出快速，高度选择性和灵敏的双色度和NIR荧光开启信号响应，并允许在存在氨基酸，生物硫醇和脂肪族硫醇的分析物存在下检测硫酚。此外，该探针在655 nm处的荧光与硫酚浓度在0到20μM之间显示出良好的线性关系，并且检测限低至12 nM。此外，