Recent studies have indicated that urinary sediment miRNAs not only are able to serve as non-invasive diagnostic biomarkers for IgA nephropathy (IgAN) but may also be closely related to several clinical and pathological indicators. However, the lack of a suitable internal reference miRNA has hampered research into urinary sediment miRNAs. To date, U6 has been used as a reference gene in urinary sediment miRNA studies mostly based on the results from studies using tissue samples and cell lines. In a total of 330 IgAN patients, 164 disease control patients and 130 normal control patients, there was no significant difference in U6 levels. We also compared the U6 levels in different types of primary glomerular disease groups (IgA nephropathy, membranous nephropathy, minimal change nephrosis and focal segmental glomerular sclerosis). The results confirmed that there was no significant difference in the expression of U6 in different primary glomerular disease groups. Moreover, treatment had no significant effect on the expression levels of U6 in IgA nephropathy. Therefore, U6 is an excellent housekeeping gene for urinary sediment miRNA studies of IgA nephropathy.

中文翻译：

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U6可作为管家基因用于IgA肾病的尿沉渣miRNA研究。

最近的研究表明，尿沉渣miRNAs不仅可以作为IgA肾病（IgAN）的非侵入性诊断生物标志物，而且还可能与几种临床和病理指标密切相关。但是，缺乏合适的内部参照miRNA阻碍了对尿沉渣miRNA的研究。迄今为止，U6已被用作尿沉渣miRNA研究中的参考基因，主要是基于组织样本和细胞系研究的结果。在总共330名IgAN患者，164名疾病控制患者和130名正常对照患者中，U6水平没有显着差异。我们还比较了不同类型的原发性肾小球疾病组（IgA肾病，膜性肾病，微小改变性肾病和局灶性节段性肾小球硬化）的U6水平。结果证实，在不同的原发性肾小球疾病组中，U6的表达没有显着差异。而且，治疗对IgA肾病中U6的表达水平没有显着影响。因此，U6是用于IgA肾病尿沉渣miRNA研究的优秀管家基因。