Rhodotorula glutinis, an oleaginous red yeast, intrinsically produces several bio-products (i.e., lipids, carotenoids and enzymes) and is regarded as a potential host for biorefinery. In view of the limited available genetic engineering tools for this yeast, we have developed a useful genetic transformation method and transformed the β-carotene biosynthesis genes (crtI, crtE, crtYB and tHMG1) and cellulase genes (CBHI, CBHII, EgI, EgIII, EglA and BGS) into R. glutinis genome. The transformant P4-10-9-63Y-14B produced significantly higher β-carotene (27.13 ± 0.66 mg/g) than the wild type and also exhibited cellulase activity. Furthermore, the lipid production and salt tolerance ability of the transformants were unaffected. This is the first study to engineer the R. glutinis for simultaneous β-carotene and cellulase production. As R. glutinis can grow in sea water and can be engineered to utilize the cheaper substrates (i.e. biomass) for the production of biofuels or valuable compounds, it is a promising host for biorefinery.

中文翻译：

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工程化含油的红色酵母Rhodotorula glutinis，以同时生产β-胡萝卜素和纤维素酶。

Rhodotorula glutinis，一种油质红色酵母，本质上产生几种生物产物（即脂质，类胡萝卜素和酶），被认为是生物精炼的潜在宿主。鉴于该酵母的可用基因工程工具有限，我们开发了一种有用的遗传转化方法，并转化了β-胡萝卜素生物合成基因（crtI，crtE，crtYB和tHMG1）和纤维素酶基因（CBHI，CBHII，EgI，EgIII， EglA和BGS）转化为R.glutinis基因组。转化体P4-10-9-63Y-14B产生的β-胡萝卜素明显高于野生型（27.13±0.66 mg / g），还表现出纤维素酶活性。此外，转化体的脂质产生和盐耐受能力不受影响。这是首次工程化谷氨酸棒杆菌同时生产β-胡萝卜素和纤维素酶的研究。作为R。