Abstract Bacteriohopanepolyols (BHPs) are characterized by a large structural diversity, although methodological constraints have limited investigations of the occurrence of isomers among composite BHPs in environmental samples. Here, we describe a novel chromatography method that uses three serial Phenomenex Kinetex C18 columns to successfully resolve new structural isomers of BHPs commonly observed in marine sediment samples. The investigated samples consistently contain a high diversity of BHP isomers, but their relative abundances differ significantly between samples. These differences in relative abundance have potential to reflect different environmental conditions such as depositional setting or redox conditions. The improved BHP resolution and baseline separation of the new method is promising for accurate quantification and future environmental proxy development and compound-specific isotope work.

中文翻译：

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改进的色谱法揭示了海洋沉积物中多种新的细菌hopane多元醇异构体

摘要 细菌hopanepolyols (BHPs) 的特点是结构多样性很大，尽管方法学上的限制限制了环境样品中复合BHPs 异构体发生的研究。在这里，我们描述了一种新的色谱方法，该方法使用三个系列 Phenomenex Kinetex C18 色谱柱成功解析了海洋沉积物样品中常见的 BHP 的新结构异构体。研究的样品始终包含高度多样性的 BHP 异构体，但它们的相对丰度在样品之间存在显着差异。这些相对丰度的差异有可能反映不同的环境条件，例如沉积环境或氧化还原条件。