Recent evidence suggests that cannabidiol (CBD) may be useful for the treatment of different neuropsychiatric disorders. However, some controversy regarding its profile as a drug of abuse hampers the further development of basic and clinical studies. In this study, the behavioral profile of CBD as a potential drug of abuse was evaluated in C57BL/6J mice. Reinforcing properties of CBD (15, 30, and 60 mg/kg; i.p.) were assessed using the conditioned place preference (CPP) paradigm. Spontaneous withdrawal symptoms and motor activity in the open field were examined 12 h after the last CBD administration (30 mg/kg/12 h, i.p., 6 days). CBD plasma concentrations were measured at 2, 4, 8, 12, and 24 h after the administration of CBD (30 mg/kg, i.p.). Furthermore, an oral CBD self-administration paradigm (50 mg/kg; CBD water-soluble 1.2 mg/mL) was performed to evaluate whether this drug produced any effects on motivation compared with a non-reinforcing substance (water). We found that CBD failed to induce CPP, withdrawal symptoms, or altered motor behavior 12 h after its administration. At that time, only traces of CBD were detected, ensuring that the lack of alterations in somatic signs and locomotor activity was not due to residual drug in plasma. Interestingly, mice displayed similar motivation and consumption of CBD and water. Taken together, these results show that CBD lacks activity as a drug of abuse and should stimulate the development of the basic and clinical studies needed to elucidate its potential therapeutic use for the treatment of neuropsychiatric and drug use disorders.

中文翻译：

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大麻二酚在小鼠行为中没有显示出药物滥用的可能性。

最近的证据表明，大麻二酚 (CBD) 可能有助于治疗不同的神经精神疾病。然而，关于其作为滥用药物的一些争议阻碍了基础和临床研究的进一步发展。在这项研究中，在 C57BL/6J 小鼠中评估了 CBD 作为潜在滥用药物的行为特征。使用条件位置偏好 (CPP) 范式评估了 CBD (15、30 和 60 mg/kg；ip) 的增强特性。在最后一次 CBD 给药 (30 mg/kg/12 h, ip, 6 天) 后 12 小时检查开放场中的自发戒断症状和运动活动。在 CBD (30 mg/kg, ip) 给药后 2、4、8、12 和 24 小时测量 CBD 血浆浓度。此外，口服 CBD 自我给药范例（50 mg/kg；CBD 水溶性 1。2 mg/mL）用于评估与非强化物质（水）相比，这种药物是否对动机产生任何影响。我们发现 CBD 在给药后 12 小时未能诱发 CPP、戒断症状或运动行为改变。当时，仅检测到微量的 CBD，确保体细胞体征和运动活动没有改变不是由于血浆中残留的药物。有趣的是，老鼠表现出相似的动机和消耗 CBD 和水。总之，这些结果表明 CBD 缺乏作为滥用药物的活性，应该促进基础和临床研究的发展，以阐明其在治疗神经精神和药物使用障碍方面的潜在治疗用途。