Hypothesis

Bolaamphiphiles are characterized by wide polymorphism of their aggregates, due to the connection of the headgroups that renders their investigation very intriguing in several technological applications. Some bolaamphiphiles displaying the triphenylphosphonium motif (TPP-bolaamphiphiles) were previously explored for their ability in crossing the mitochondrial membranes but their colloidal features, which are crucial for the potential development of an effective drug delivery system, were never investigated.

Experiments

Single chain TPP-bolaamphiphiles, featuring chains of 12, 16, 20 and 30 methylene units, were synthesized and their aggregation features (Krafft point, cac, dimensions, morphology, stability) were investigated by conductivity, dialysis, transmission electron microscopy, Raman spectroscopy, dynamic and dielectrophoretic laser light scattering measurements.

Findings

All the TPP-bolaamphiphiles spontaneously self-assemble into vesicles, independently of the chain length. The bolaamphipile with the longest chain forms monodispersed vesicles whereas for the other bolaamphiphiles two distinct populations of vesicles are observed. All vesicles are not equilibrium systems, in particular vesicles formed by the bolaamphiphiles featuring 20 and 30 methylene units result notably stable to dilution thanks to both the tightening of molecular packing at increasing chain length and the progressive reduction of the monomer percentage in U-shaped conformation.

These features make these TPP-bolaamphiphiles very attractive as minor components for the development of novel mitochondriotropic liposomes.

中文翻译：

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三苯基phosph硼两亲物的聚集行为

假设

由于头基的连接，Bolaamphiphiles的特征是其聚集体具有广泛的多态性，这使他们的研究在几种技术应用中引起了极大的兴趣。以前曾研究过一些显示三苯基phosph基序的双亲两性亲子（TPP-双亲两性亲子）具有穿越线粒体膜的能力，但从未研究过它们对于有效药物递送系统的潜在发展至关重要的胶体特征。

实验

合成了具有12个，16个，20个和30个亚甲基单元的单链TPP-双亲亲脂，并通过电导率，渗析，透射电子显微镜，拉曼光谱研究了它们的聚集特征（卡夫点，cac，尺寸，形态，稳定性）。 ，动态和介电泳激光散射测量。

发现

所有的TPP-双亲双亲分子自发地自组装成囊泡，而与链长无关。具有最长链的boampamphipile形成单分散的囊泡，而对于其他bulaamphiphiles，则观察到两个不同的囊泡群体。所有的囊泡都不是平衡系统，特别是由于具有增加的链长的分子堆积和在U形构象中单体百分比的逐渐降低，由具有20和30个亚甲基单元的双性亲脂分子形成的囊泡对稀释的结果非常稳定。 。

这些特征使这些TPP-双亲两性杆菌作为开发新型线粒体脂质体的次要成分非常有吸引力。