Fabrication of strontium/calcium containing poly(γ-glutamic acid) – organosiloxane fibrous hybrid materials for osteoporotic bone regeneration,RSC Advances

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Fabrication of strontium/calcium containing poly(γ-glutamic acid) – organosiloxane fibrous hybrid materials for osteoporotic bone regeneration
RSC Advances ( IF 3.9 ) Pub Date : 2018-07-18 00:00:00 , DOI: 10.1039/c8ra03957g
Chunxia Gao ₁ , Ke Zhao ₁ , Yaping Wu ₁ , Qiang Gao _{1,

2} , Peizhi Zhu ₁

Affiliation

Recent researches have proved that combination of several therapeutic metal ions, such as silicate (Si), calcium (Ca), strontium (Sr) and so on, with biomaterials may have promising effects for stimulating bone regeneration. In the present study, the Sr/Ca containing silicate hybrid materials (Sr/Ca-γ-PGA-silica) with a mimetic native extracellular matrix (ECM) structure have been developed by electrospinning. With the aim to promote the solubility of γ-PGA in aqueous-based solution and introduce Sr/Ca elements into the prepared hybrid materials, SrCO₃ and CaCO₃ were employed due to their nontoxicity and no by-products during chemical reaction between γ-PGA and SrCO₃/CaCO₃. Results of SEM, EDX and elemental mapping images showed that Sr and Ca have been successfully incorporated into the prepared fibrous hybrid materials with homogeneous dispersion. Results of ICP-AES revealed that there was continuous Si, Sr and Ca ion release behavior of Sr/Ca-γ-PGA-silica hybrid materials in Tris–HCl buffer solution and the Si ions release rate can be tailored by adjusting the molar ratio of Sr to Ca. Immersion of Sr/Ca-γ-PGA-silica hybrid materials in a simulated body fluid (SBF) resulted in the formation of an apatite-like surface layer within 3 days, indicating their excellent bioactivity. In addition, the prepared Sr/Ca-γ-PGA-silica hybrid materials supported the proliferation and alkaline phosphatase (ALP) activity of osteoblast in vitro, showing their good biocompatibility. Altogether, the results indicated that the prepared Sr/Ca-γ-PGA-silica hybrid materials with an adjusted ionic release behavior have great potential for providing an excellent ECM for osteoporotic bone regeneration.

中文翻译：

用于骨质疏松性骨再生的含锶/钙的聚（γ-谷氨酸）-有机硅氧烷纤维杂化材料的制备

最近的研究表明，硅酸盐（Si）、钙（Ca）、锶（Sr）等几种治疗性金属离子与生物材料的结合可能对刺激骨再生具有良好的效果。在本研究中，通过静电纺丝技术开发了具有模拟天然细胞外基质 (ECM) 结构的含 Sr/Ca 硅酸盐杂化材料 ( Sr/Ca-γ-PGA-silica )。为了提高γ-PGA在水基溶液中的溶解度并将 Sr/Ca 元素引入制备的杂化材料中，由于 SrCO ₃和 CaCO ₃无毒且在γ-之间的化学反应过程中没有副产物，因此被采用PGA和SrCO ₃ /CaCO₃ . SEM、EDX 和元素映射图像的结果表明，Sr 和 Ca 已成功地掺入到所制备的纤维杂化材料中，并具有均匀的分散性。ICP-AES结果表明， Sr/Ca-γ-PGA-二氧化硅杂化材料在Tris-HCl缓冲溶液中存在连续的Si、Sr和Ca离子释放行为，并且可以通过调节摩尔比来调整Si离子释放速率。从 Sr 到 Ca。将Sr/Ca-γ-PGA-二氧化硅杂化材料浸入模拟体液 (SBF) 中，可在 3 天内形成类似磷灰石的表面层，表明它们具有优异的生物活性。此外，制备的Sr/Ca-γ-PGA-silica杂化材料在体外支持成骨细胞的增殖和碱性磷酸酶（ALP）活性，表现出良好的生物相容性。总之，结果表明，制备的具有调节离子释放行为的Sr/Ca-γ-PGA-二氧化硅杂化材料具有为骨质疏松性骨再生提供优良 ECM 的巨大潜力。

更新日期：2018-07-18

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