Abstract The combined effect of thymol, carvacrol and mild heat treatments against Listeria monocytogenes Scott A was assessed in buffered system by plate counting and flow cytometry (FCM). The susceptibility of cells increased when heat treatment was combined with terpenes. The data modelling with Bigelow and Weibull equations showed that the latter was more appropriate to describe the inactivation kinetics. After treatments, cells were no longer able to form colonies on plates; nevertheless, FCM indicated that most of the cells were damaged rather than dead. Treated cells were not able to recover the damage after 6 h. This opens the question if they could recover in a food matrix. FCM can be a helpful technique to better comprehend the physiological state of microorganisms. In the perspective of industrial applications, studies based on predictive microbiology, as well as a deeper comprehension of the action mechanisms of antimicrobials, play a key role for process optimization.

中文翻译：

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在百里酚和香芹酚存在下温和热处理后单核细胞增生李斯特菌 Scott A 的建模：对可培养性和生存力的影响

摘要 通过平板计数和流式细胞术 (FCM) 在缓冲系统中评估百里酚、香芹酚和温和热处理对单核细胞增生李斯特菌 Scott A 的综合作用。当热处理与萜烯结合时，细胞的敏感性增加。Bigelow 和 Weibull 方程的数据建模表明后者更适合描述失活动力学。处理后，细胞不再能够在平板上形成集落；尽管如此，FCM 表明大多数细胞受损而不是死亡。处理过的细胞在 6 小时后无法恢复损伤。这提出了它们是否可以在食物基质中恢复的问题。FCM 是一种有助于更好地了解微生物生理状态的技术。从工业应用的角度来看，