AbstractNatural polysaccharides are an important class of biomaterials that have attracted significant research interest for biomedical applications because of their high biocompatibility, biodegradability, and bioactivity. In this work, we fabricated water-insoluble composite hollow and solid fibers made of polyion complexes of chondroitin sulfate C (CS) and chitosan (CHI) using a single microfluidic device. A coaxial two-phase microfluidic device was constructed from stainless-steel needles and glass fibers, and CHI solutions and CS solutions were continuously infused into the core and sheath channels, respectively. The obtained fibers were flexible and homogeneous and had diameters of a few hundred micrometers. Hollow fibers were formed using water as the solvent of CS, while core-filled solid fibers were obtained using 20% (v/v) ethanol. The respective mechanisms for the fabrication of the hollow and solid fibers were discussed. An increase in the sheath flow rate or decrease in the core flow rate reduced the diameters of the fibers, while a reduction in the concentration of the CHI solution reduced the thickness of the hollow fibers. Furthermore, bovine serum albumin, used as a model protein, could be incorporated in the hollow and solid fibers by mixing them in the core flow solution. These results suggested the great potential of microfluidic techniques for the preparation of hollow and solid polysaccharide fibers.Hollow and solid fibers made of polyion complexes of chondroitin sulfate C (CS) and chitosan (CHI) were selectively fabricated using a coaxial two-phase microfluidic device. The selective fabrication was successfully achieved only by changing the solvent of the sheath flow that contained CS. Proteins and nanomaterials could be incorporated in both fibers by mixing them in the core flow, CHI-containing solutions. Results suggested the great potentials of microfluidic techniques for preparation of polysaccharide hollow and solid fibers.

中文翻译：

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使用微流体装置通过控制聚离子复合物的形成选择性制造空心和实心多糖复合纤维

摘要 天然多糖是一类重要的生物材料，由于其良好的生物相容性、生物降解性和生物活性，在生物医学应用方面引起了广泛的研究兴趣。在这项工作中，我们使用单个微流体装置制造了由硫酸软骨素 C (CS) 和壳聚糖 (CHI) 的聚离子复合物制成的水不溶性复合空心和实心纤维。同轴两相微流体装置由不锈钢针和玻璃纤维构成，CHI 溶液和 CS 溶液分别连续注入核心和鞘通道。获得的纤维是柔韧且均匀的并且具有几百微米的直径。使用水作为 CS 的溶剂形成中空纤维，而使用 20% (v/v) 乙醇获得填充芯的固体纤维。讨论了制造空心和实心纤维的各自机制。鞘流率的增加或芯流率的降低减小了纤维的直径，而CHI溶液浓度的降低减小了中空纤维的厚度。此外，用作模型蛋白质的牛血清白蛋白可以通过将它们混合在核心流动溶液中而掺入中空纤维和实心纤维中。这些结果表明微流控技术在制备空心和实心多糖纤维方面具有巨大潜力。 使用同轴两相微流控装置选择性地制造了由硫酸软骨素 C (CS) 和壳聚糖 (CHI) 的聚离子复合物制成的空心和实心纤维. 只有通过改变包含 CS 的鞘流的溶剂才能成功实现选择性制造。蛋白质和纳米材料可以通过将它们混合在含有 CHI 的核心流中的溶液中混合到两种纤维中。结果表明微流体技术在制备多糖中空和实心纤维方面具有巨大的潜力。