Cancer is one of the main causes of death in today's world, therefore, extending fast and reliable approaches for early monitoring of cancer cells have become one of the great challenges for scientists. This work reports exploiting a fast single frequency impedance strategy coupled to a new constructed composite for the quantitative detection of the human chronic myelogenous leukemia (CML) cell lines, K562. The platform used for the fabrication of the cytosensor is based on an electrospun poly[ethersulfone] (PES) sub-layer ready for improving by the upper nanodimension layers comprised of gold nanoparticles and multiwall carbon nanotubes (AuNPs/MWCNTs). The binding of K562 cancer cells with the pre-immobilized capture ssDNA was successfully transduced by Faradaic impedance spectroscopy (FIS). To diminish the possible nonspecific impedance changes that may cause some uncertainties in FIS signal, and to considerably decrease the time of the extended method, a fast single frequency measurement (SFM) strategy was tested based on recording total impedance |Z| in different individual frequencies, the methodology that was succeeded to recognize the target cells in measurement times as low as several seconds. Under the optimum conditions, the assessed signals were proportional to the population of K562 cells from 10² to 10⁷ cells mL⁻¹ with a calculated detection limit of 60 cells mL⁻¹. The proposed biosensing-device also demonstrated high stability and reproducibility, which was able to offer great promise for the quantitative assay of K562 in routine analyses. The results of real sample analyses introduced the proposed device as alternative tool for CML K562 cell detection in real biological samples.

癌症是当今世界的主要死亡原因之一，因此，扩展用于早期监测癌细胞的快速可靠的方法已成为科学家面临的重大挑战之一。这项工作报告利用快速单频阻抗策略与新型复合材料相结合，用于定量检测人类慢性粒细胞白血病（CML）细胞系K562。用于制造细胞传感器的平台基于静电纺丝的聚醚砜（PES）子层，可以通过由金纳米颗粒和多壁碳纳米管（AuNPs / MWCNTs）组成的上部纳米尺寸层进行改进。通过法拉第阻抗谱（FIS）成功地转导了K562癌细胞与预先固定的捕获ssDNA的结合。为了减少可能引起FIS信号不确定性的非特定阻抗变化，并显着减少扩展方法的时间，基于记录总阻抗| Z |进行了快速单频测量（SFM）策略的测试。在不同的个体频率下，成功地以低至几秒钟的测量时间识别目标细胞的方法。在最佳条件下，评估的信号与来自10个细胞的K562细胞的数量成正比。该方法成功地以低至几秒钟的测量时间识别了靶细胞。在最佳条件下，评估的信号与来自10个细胞的K562细胞的数量成正比。该方法成功地以低至几秒钟的测量时间识别了靶细胞。在最佳条件下，评估的信号与来自10个细胞的K562细胞的数量成正比。²至10 ^7个 细胞mL ^-1，计算出的检出限为60个细胞mL ^-1。所提出的生物传感装置还显示出高稳定性和可重复性，这能够为常规分析中K562的定量测定提供广阔的前景。真实样品分析的结果介绍了拟议的设备，作为在真实生物样品中检测CML K562细胞的替代工具。