In this study, two novel binuclear Co(II) complexes, [Co₄(L₁)₂(CH₃CH₂O)₄·5.5H₂O (1) and [Co₂(L₂)₂(CH₃COO)(H₂O)]·2CH₃OH·3H₂O (2)], with the ligands H₃L₁ and H₂L₂ were synthesized and structurally characterized using single crystal X-ray diffraction. The crystal structures of complex 1 and 2 belong to a monoclinic system with space group of P2(1)/n and triclinic system with space group of P-1, respectively. Their interaction with CT-DNA was investigated using electron absorption and fluorescent spectroscopy. The apparent binding constants (K_app) of two complexes (1 and 2) were determined to be 5.34 × 10⁵ and 5.82 × 10⁵, respectively, and both exhibited a moderate intensity of insertion with 2 > 1. Fluorescence quenching studies indicated that the fluorescence quenching mechanism of complex 1 is dynamic quenching and that of complex 2 is a static quenching mechanism. The chemical nuclease activity of the complexes was studied using agarose gel electrophoresis. The results suggest that when H₂O₂ is added, complexes 1 and 2 exhibit chemical nuclease activity. The cleavage mechanisms between the complexes and plasmid DNA are the likely oxidative cleavage process of hydroxyl radical (OH) and singlet oxygen (¹O₂) as active species.

在这项研究中，两个新颖的双核Co（II）配合物，[Co ₄（L ₁）₂（CH ₃ CH ₂ O）₄ ·5.5H ₂ O（1）和[Co ₂（L ₂）₂（CH ₃ COO） ）（H ₂ O）]·2CH ₃ OH·3H ₂ O（2）]与配体H ₃ L ₁和H ₂ L ₂合成，并通过单晶X射线衍射进行结构表征。配合物1和2的晶体结构分别属于空间群为P2（1）/ n的单斜晶系和空间群为P-1的三斜晶系。使用电子吸收和荧光光谱研究了它们与CT-DNA的相互作用。确定两个复合物（1和2）的表观结合常数（K _app）分别为5.34×10 ⁵和5.82×10 ⁵，并且都显示出适中的插入强度，其中2  >  1。荧光猝灭研究表明配合物1的荧光猝灭机理是动态猝灭，配合物2的荧光猝灭机理是动态猝灭。是静态淬灭机制。使用琼脂糖凝胶电泳研究复合物的化学核酸酶活性。结果表明，当添加H ₂ O _2时，复合物1和2表现出化学核酸酶活性。复合物与质粒DNA之间的裂解机理是作为活性物种的羟基自由基（OH）和单线态氧（¹ O ₂）可能的氧化裂解过程。