Industrial development of microalgae biomass valorization relies on process optimization and controlled scale-up. Both need robust modeling: (i) for biomass production and (ii) for integrated processes in the downstream processing (DSP). Cell disruption and primary fractionation are key steps in DSP. In this study, a kinetic model, including microalgal cell size distribution, was developed for Chlorella sorokiniana disruption in continuous bead milling. Glass beads of 0.4 mm size at impeller tip velocity of 14 m.s⁻¹ were used as optimal conditions for efficient cell disruption. These conditions allowed faster disruption of big cells than small ones. A modified expression of the Stress Number, including cell size effect, was then proposed and validated.

Separation of starch, proteins and chlorophyll by mild centrifugation was studied as function of the disruption parameters. Low energy consumption conditions led to extreme comminution. An intermediate zone drew attention for allowing moderate energy consumption and efficient metabolites separation by centrifugation.

微藻生物量评估的工业发展依赖于工艺优化和可控的规模扩大。两者都需要鲁棒的建模：（i）用于生物质生产，（ii）用于下游处理（DSP）中的集成过程。细胞分裂和初级分离是DSP中的关键步骤。在这项研究中，建立了动力学模型，包括微藻细胞大小分布，用于在连续珠磨中破坏小球藻。在叶轮尖端速度为14 ms ^-1时，将0.4毫米大小的玻璃珠用作有效细胞破碎的最佳条件。这些条件允许大细胞比小细胞更快地破裂。然后提出并验证了应力数的修饰表达，包括细胞大小效应。

研究了通过温和离心分离淀粉，蛋白质和叶绿素与破坏参数的关系。低能耗条件导致极度粉碎。一个中间区域引起了人们的注意，它允许适度的能量消耗和通过离心分离有效的代谢产物。