Short-term glucose starvation prior to chemotherapy has the potential to preferentially weaken cancer cells, making them more likely to succumb to treatment, while protecting normal cells. In this study, we used 3D cell cultures of colorectal cancer and assessed the effects of short-term glucose starvation and chemotherapy compared to treatment of either individually. We evaluated both phenotypic changes and protein expression levels. Our findings indicate that the combined treatment results in more significant phenotypic responses, including decreased cell viability and clonogenicity. These phenotypic responses can be explained by the decreased expression of LDHA and 14-3-3 family proteins, which were found only in the combined treatment groups. This study indicates that short-term glucose starvation has the potential to increase the efficacy of current cancer treatment regimes. Graphical Abstract ᅟ.

中文翻译：

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3D 结直肠癌细胞培养中联合短期葡萄糖饥饿和化疗可降低 14-3-3 家族蛋白表达和对治疗的表型反应。


化疗前的短期葡萄糖饥饿有可能优先削弱癌细胞，使它们更有可能死于治疗，同时保护正常细胞。在这项研究中，我们使用了结直肠癌的 3D 细胞培养物，并评估了短期葡萄糖饥饿和化疗与单独治疗的效果。我们评估了表型变化和蛋白质表达水平。我们的研究结果表明，联合治疗会导致更显着的表型反应，包括细胞活力和克隆性降低。这些表型反应可以通过 LDHA 和 14-3-3 家族蛋白表达的降低来解释，这些蛋白仅在联合治疗组中发现。这项研究表明，短期葡萄糖饥饿有可能提高当前癌症治疗方案的疗效。图形摘要ᅟ。