Herein, a doped graphene-like membrane was designed, which was copolymerized to be a solid ionic biosensor by using titania nanotubes (TiNTs), polyaniline (PANI), EvimCl (1-ethyl-3-vinylimidazolium chloride, EVIMC) and chloroauric acid (HAuCl₄). The structure of graphene-like arrangement and the copolymerization mechanism of the film were discussed in detail. Because of the high catalytic property, Lactate could be determined on the membrane catalyzed by lactate dehydrogenase (LDH) containing isocitrate dehydrogenase (NAD⁺), which was immobilized onto the film by electrostatic attraction. The electrochemical response on the LDH/Au-EVIMC-TiNTs-PANI/ITO was increased by twice than the LDH/TiNTs/PANI/ITO, and exhibited two linear responses within the concentration range from 5.5 × 10⁻⁷ M to 5.55 × 10⁻⁶ M, and 5.55 × 10⁻⁶ M to 3.33 × 10⁻³ M, with a detection limit of 1.65 × 10⁻⁷ M (S / N = 3). The interference study for other common coexistence such as uric acid and hemoglobin revealed that there was no overlapping signal for the detection of Lactate on the biosensor. The developed method proved the most stability in the determination of real blood samples, and the recoveries ranged from 96.7% to 105.8% with a satisfactory result.

本文设计了一种掺杂石墨烯的膜，该膜通过使用二氧化钛纳米管（TiNTs），聚苯胺（PANI），EvimCl（1-乙基-3-乙烯基咪唑鎓氯化物，EVIMC）和氯金酸（ HAuCl ₄）。详细讨论了石墨烯状结构的结构和薄膜的共聚机理。由于具有较高的催化性能，因此可以通过含有异柠檬酸脱氢酶（NAD ⁺）的乳酸脱氢酶（LDH）催化的膜来确定乳酸盐，该乳酸盐通过静电吸引固定在薄膜上。LDH / Au-EVIMC-TiNTs-PANI / ITO的电化学响应是LDH / TiNTs / PANI / ITO的两倍，在5.5×10的浓度范围内表现出两个线性响应^-7 M至5.55×10 ^-6 M和5.55×10 ^-6 M至3.33×10 ^-3 M，检测极限为1.65×10 ^-7 M（S / N  = 3）。对其他常见共存物（例如尿酸和血红蛋白）的干扰研究表明，在生物传感器上检测乳酸没有重叠信号。所开发的方法证明了在测定真实血液样品中最稳定，回收率在96.7％至105.8％之间，结果令人满意。