Signal-amplified ratiometric electrochemiluminescence (Sa-RECL) provides an attractive approach to maximize signal-to-noise ratio through enhancing signals and eliminate interferences. In this work, we prepared a novel metal-organic framework (MOF)/Au/G-quadruplex as both quenchers and enhancers to fabricate a target-triggered ratiometric ECL sensor for high sensitive and accurate detection of prostatic specific antigen (PSA). The ratiometric ECL sensor using the dual-potential-dependent ECL emitters (quantum dots (QDs) and luminol) and MOF/Au/G-quadruplex not only achieved signal self-calibration but also realized cooperative amplification. After the sequential hybridization among of complementary DNA-QDs, PSA aptamer and pDNA-Au-Hemin-MIL-DNAzyme and the further competition of PSA, the pDNA-Au-Hemin-MIL-DNAzyme probe would keep away from the electrode surface, causing a switchover of their ECL signals from “off-on” state to “on-off” state. The ratiometric ECL aptasensor exhibits high-sensitive and accurate analytical performance toward PSA with a linear detection range from 0.5 to 500 ng mL⁻¹ and a detection limit of 0.058 ng mL⁻¹ (S/N = 3). The novel ratiometric ECL biosensor has been successfully applied to determine different serum samples of PSA, indicating its potential application in the clinical diagnosis.

信号放大比例电化学发光（Sa-RECL）提供了一种有吸引力的方法，可通过增强信号来最大程度地提高信噪比并消除干扰。在这项工作中，我们准备了一种新型的金属有机骨架（MOF）/ Au / G-四链体作为淬灭剂和增强剂，以制造目标触发的比例式ECL传感器，用于高灵敏，准确地检测前列腺特异性抗原（PSA）。使用依赖于双电势的ECL发射器（量子点（QD）和鲁米诺）和MOF / Au / G四联体的比例ECL传感器不仅实现了信号自校准，而且实现了协同放大。在互补DNA-QD，PSA适体和pDNA-Au-Hemin-MIL-DNAzyme之间进行顺序杂交后，PSA进一步竞争，pDNA-Au-Hemin-MIL-DNAzyme探针会远离电极表面，从而导致其ECL信号从“关闭-开启”状态切换到“开启-关闭”状态。比例式ECL适体传感器对PSA表现出高灵敏且准确的分析性能，线性检测范围为0.5至500 ng mL^-1，检测极限为0.058 ng mL ^-1（S / N = 3）。新型比例式ECL生物传感器已成功应用于确定PSA的不同血清样品，表明其在临床诊断中的潜在应用。