Molecularly imprinted polymers (MIPs) can selectively bind target molecules and can therefore be advantageously used as a low-cost and robust alternative to replace fragile and expensive natural receptors. Yet, one major challenge in using MIPs for sensor development is the lack of simple and cost-effective techniques that allow firm fixation as well as controllable and consistent receptor material distribution on the sensor substrate. In this work, a convenient method is presented wherein microfluidic systems in conjunction with in situ photo-polymerization on functionalized diamond substrates are used. This novel strategy is simple, efficient, low-cost and less time consuming. Moreover, the approach ensures a tunable and consistent MIP material amount and distribution between different sensor substrates and thus a controllable active sensing surface. The obtained patterned MIP structures are successfully tested as a selective sensor platform to detect physiological concentrations of the hormone disruptor testosterone in buffer, urine and saliva using electrochemical impedance spectroscopy. The highest added testosterone concentration (500 nM) in buffer resulted in an impedance signal of 10.03 ± 0.19% and the lowest concentration (0.5 nM) led to a measurable signal of 1.8 ± 0.15% for the MIPs. With a detection limit of 0.5 nM, the MIP signals exhibited good linearity between a 0.5 nM and 20 nM concentration range. Apart from the excellent and selective recognition offered by these MIP structures, they are also stable during and after the dynamic sensor measurements. Additionally, the MIPs can be easily regenerated by a simple washing procedure and are successfully tested for their reusability.

分子印迹聚合物（MIP）可以选择性地结合目标分子，因此可以有利地用作替代脆弱而昂贵的天然受体的低成本且健壮的替代品。然而，使用MIP进行传感器开发的一个主要挑战是缺乏简单且具有成本效益的技术，这些技术无法牢固固定，并且在受体基质上的受体材料分布可控且一致。在这项工作中，提出了一种方便的方法，其中使用微流体系统结合在功能化金刚石基材上进行原位光聚合。这种新颖的策略简单，高效，低成本且耗时少。而且，该方法可确保在不同传感器基板之间可调节且一致的MIP材料数量和分布，从而可控制主动感应表面。获得的图案化MIP结构已成功测试为选择性传感器平台，可使用电化学阻抗光谱法检测缓冲液，尿液和唾液中激素干扰素睾丸激素的生理浓度。缓冲液中最高添加的睾丸激素浓度（500 nM）导致阻抗信号为10.03±0.19％，最低浓度（0.5 nM）导致MIP的可测量信号为1.8±0.15％。检测限为0.5 nM，MIP信号在0.5 nM和20 nM浓度范围之间表现出良好的线性。除了这些MIP结构提供的出色和选择性的认可外，在动态传感器测量期间和之后，它们也很稳定。此外，MIP可以通过简单的清洗程序轻松再生，并已成功测试了它们的可重复使用性。