In the present study, an acute toxic test was performed to assess the oxidative stress and genotoxic effects of the herbicide on the freshwater shrimp Macrobrachium nipponensis. The results showed that the 48-h and 96-h LC₅₀ values of Roundup to M. nipponensis were 57.684 mg/L and 11.237 mg/L, respectively. For further investigation, the shrimps were exposed to sublethal concentrations of 0.35, 0.70, 1.40, 2.80 and 5.60 mg/L for 96 h. A significant decrease in total haemocytes count (THC) was observed at concentration of 5.60 mg/L throughout the experiment. The level of superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (T-AOC) in all the treatments decreased in a dose- and time-dependent manner except for the concentration group of 0.35 mg/L. The malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and protein carbonyl in serum increased significantly at concentrations of 2.80 mg/L and 5.60 mg/L. A significant decrease in acetylcholinesterase (AChE) activity was observed at each concentration (P＜0.05). In addition, the micronucleus (MN) frequency of haemocytes significantly increased (P＜0.05) at concentrations of 1.40, 2.80 and 5.60 mg/L, whereas the comet ratio and %DNA in the tails exhibited a clear time- and dose-dependent response during the exposure. The analysis of the integrated biomarker response (IBR) showed the induction of oxidative stress biomarkers and the inhibition of antioxidants, and this dose-dependent relation suggests the sensitivity and availability of all the biomarkers. These results revealed that Roundup had a prominent toxic effect on M. nipponensis based on the antioxidative response inhibition and genotoxicity.

在本研究中，进行了急性毒性试验，以评估除草剂对淡水虾日本沼虾的氧化应激和基因毒性作用。结果表明，农达对日本稻瘟病菌的48小时和96小时LC ₅₀值分别为57.684 mg/L和11.237 mg/L。为了进一步研究，将虾暴露于 0.35、0.70、1.40、2.80 和 5.60 mg/L 的亚致死浓度下 96 小时。在整个实验过程中，浓度为 5.60 mg/L 时观察到总血细胞计数 (THC) 显着下降。除0.35 mg/L浓度组外，各处理中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）水平均呈剂量和时间依赖性下降。血清中丙二醛（MDA）、过氧化氢（H ₂ O ₂ ）和蛋白质羰基在2.80 mg/L和5.60 mg/L浓度下显着升高。各浓度下乙酰胆碱酯酶（AChE）活性均显着降低（ P ＜0.05）。此外，在1.40、2.80和5.60 mg/L浓度下，血细胞微核（MN）频率显着增加（ P ＜0.05），而尾部彗星比和%DNA则表现出明显的时间和剂量依赖性响应。曝光期间。综合生物标志物反应（IBR）的分析表明氧化应激生物标志物的诱导和抗氧化剂的抑制，这种剂量依赖性关系表明所有生物标志物的敏感性和可用性。这些结果表明农达对结核分枝杆菌具有显着的毒性作用。 nipponensis 基于抗氧化反应抑制和遗传毒性。