To enhance nitrogen removal efficiency, a new electrolysis augmented constructed wetland (E-CW) was applied for nitrogen removal from waste water treatment plant (WWTP) effluent. This work demonstrated that E-CW could remove NO₃⁻ efficiently (45.5%–83.4%) under low C_COD/N ratio (average 2.29 ± 0.45) with little amount of NH₄⁺ and NO₂⁻ generation. High throughput 16S rRNA sequence analysis revealed that Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Verrucomicrobia were the dominant phyla in the E-CW. However, abundance of denitrifiers and denitrification genes decreased along with the operation of E-CW. Four functional gene pairs of anammox-amoA, (narG + napA)-(nirK + nirS), (narG + napA)-nosZ and qnorB-nosZ showed positive correlations with each other. Co-occurrence network analysis results indicated that functional guilds of FeOB and FeRB coupled with denitrifiers and contributed to the process of nitrogen removal in the E-CW. Overall, this work illustrated E-CW was a feasible and effective technology for enhancing nitrogen removal, and provided a theoretical basis for better design and operation of E-CW.

为了提高脱氮效率，新的电解增强人工湿地（E-CW）被用于从废水处理厂（WWTP）废水中脱氮。这项工作表明，E-CW可以除去NO ₃ ^-低C下有效（45.5％-83.4％）_COD / N比（平均2.29±0.45）与NH的少量₄ ⁺和NO ₂ ^-的生成。高通量16S rRNA序列分析表明，变形杆菌，放线菌，蓝细菌，拟杆菌和疣状微生物是E-CW中的主要种群。然而，随着E-CW的运行，反硝化剂和反硝化基因的丰度降低。四个功能基因对ammamoxamoA（narG  + 的napA） - （nirK  +  NIRS），（narG  + 的napA） - nosZ和qnorB - nosZ显示彼此正相关。共现网络分析结果表明，FeOB和FeRB的功能组合与反硝化剂结合，有助于E-CW中的脱氮过程。总的来说，这项工作说明了电连续水是提高氮去除率的可行和有效的技术，并为更好地设计和运行电连续水提供了理论基础。