Co₃O₄ nanoparticles homogeneously anchored on montmorillonite (MMT) were synthesized via a facile hydrothermal method. Co₃O₄-MMT were demonstrated to possess intrinsic peroxidase- and weak oxidase-like activity, and employed as peroxidase mimics for the first time. The efficient catalytic activity of the Co₃O₄-MMT NCs may originate from the electron transfer among catalyst, TMB (3,3′,5,5′-tetramethylbenzidine) and H₂O₂. Furthermore, a sensitive colorimetric sensor for glutathione based on the peroxidase activity of Co₃O₄-MMT is proposed with a low detection limit of 0.088 μM. And the proposed method had been successfully applied in detecting GSH in human serum and reduced glutathione for injection solutions.

通过简便的水热法合成了均匀锚固在蒙脱土（MMT）上的Co ₃ O ₄纳米粒子。Co ₃ O ₄ -MMT被证明具有固有的过氧化物酶和弱氧化酶样活性，并首次用作过氧化物酶模拟物。Co ₃ O ₄ -MMT NCs的有效催化活性可能源于催化剂，TMB（3,3'，5,5'-四甲基联苯胺）和H ₂ O ₂之间的电子转移。此外，基于Co ₃ O ₄的过氧化物酶活性的谷胱甘肽敏感比色传感器-MMT建议具有0.088μM的低检测限。该方法已成功地用于人血清中谷胱甘肽和还原型谷胱甘肽的检测。