Mercury (Hg) is one of the most dangerous elements, and its toxicity and ability to accumulate in organisms depend on its chemical form. There are numerous methods of Hg speciation analysis, out of which the least expensive and the least time-consuming one is thermodesorption. The method has been successfully used for the analysis of abiotic samples – soils and sediments. The aim of this study was to verify whether the simplified thermodesorption method can be used in the analysis of the tissues of animal organisms from different trophic levels. Hg fractionation analyses were performed on a DMA-80 analyser (Milestone, Italy). The results presented in this paper are the first published data on Hg fractionation by thermodesorption method in animal tissues. The study showed that the 5-step thermodesorption method can be applied to various types of environmental matrices, which makes it universal. This method is of great importance in terms of estimating the Hg uptake and transfer in the trophic chain, and also enables the assessment of global Hg circulation in the environment. The presented method does not require previous digestion of samples or the use of expensive reagents. It can also be used for the preliminary selection of samples for MeHg analysis. The results obtained by this 5-step fractionation could be comparable with different research, conducted using other Hg analysers.

汞（Hg）是最危险的元素之一，其毒性和在生物体内积累的能力取决于其化学形式。汞形态分析的方法很多，其中最便宜，最耗时的是热脱附。该方法已成功用于分析非生物样品（土壤和沉积物）。这项研究的目的是验证简化的热脱附方法是否可以用于分析来自不同营养水平的动物有机体的组织。汞分馏分析是在DMA-80分析仪（意大利米利斯顿）上进行的。本文介绍的结果是通过热解吸法在动物组织中分离汞的第一个公开数据。研究表明，五步热解吸方法可应用于各种类型的环境基质，这使其具有通用性。在估算营养链中汞的吸收和转移方面，该方法非常重要，并且还可以评估环境中全球汞的循环。提出的方法不需要事先消化样品或使用昂贵的试剂。它也可以用于MeHg分析的样品的初步选择。通过五步分级分离获得的结果可以与使用其他汞分析仪进行的不同研究相媲美。并且还可以评估环境中全球汞的循环。提出的方法不需要事先消化样品或使用昂贵的试剂。它也可以用于MeHg分析的样品的初步选择。通过五步分级分离获得的结果可以与使用其他汞分析仪进行的不同研究相媲美。并且还可以评估环境中全球汞的循环。提出的方法不需要事先消化样品或使用昂贵的试剂。它也可以用于MeHg分析的样品的初步选择。通过五步分级分离获得的结果可以与使用其他汞分析仪进行的不同研究相媲美。