AbstractA method is described for the chemiluminescence based determination of the activity of catalase (CAT) using H2O2-sensitive CdTe quantum dots (QDs). It is based on the finding that the chemiluminescence (CL) of the CdTe/H2O2 system is reduced due to the consumption of H2O2 by the catalytic action of CAT. The Michaelis constant is calculated to be 519 ± 27 mM, showing the potential of the method to accurately measure the Km compared to the standard method. The method does not require QDs to be conjugated to biological/organic molecules and therefore is considered to be a rapid and convenient method for determination of CAT in real samples. At an incubation time of 2 s, the LOD was calculated to be 4.5 unit/mL, with a linear range from 6 to 400 unit/mL. The assay is sensitive, simple, and suitable for practical applications. Graphical abstractSchematic representation of chemiluminescence-based catalase U(CAT) assay using the CdSe QD/H2O2 system. The reduction of H2O2 is reflected by the chemiluminescence of the QDs. A mechanism is put forward based on the changes in chemiluminescence intensity of the QDs by the consumption of H2O2 due to the catalytic action of CAT.

中文翻译：

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使用 H2O2 敏感 CdTe 量子点的基于化学发光的过氧化氢酶测定

摘要描述了一种使用 H2O2 敏感的 CdTe 量子点 (QD) 基于化学发光测定过氧化氢酶 (CAT) 活性的方法。它基于发现 CdTe/H2O2 系统的化学发光 (CL) 由于 CAT 的催化作用消耗了 H2O2 而降低。米氏常数计算为 519 ± 27 mM，表明与标准方法相比，该方法具有准确测量 Km 的潜力。该方法不需要将量子点与生物/有机分子结合，因此被认为是一种快速方便的测定实际样品中 CAT 的方法。在 2 s 的孵育时间，计算出的 LOD 为 4.5 unit/mL，线性范围为 6 到 400 unit/mL。该测定灵敏、简单，适合实际应用。图形摘要使用 CdSe QD/H2O2 系统的基于化学发光的过氧化氢酶 U(CAT) 测定的示意图。H2O2 的减少由 QD 的化学发光反映出来。基于CAT催化作用消耗H2O2导致QDs化学发光强度变化，提出了一种机制。