Every individual needs food for its nutritional value and flavor while the economic growth of a nation depends on a thriving profit-generating industry. The food industry caters to both needs in an efficient manner. Proteins can rightly be considered as the driving force behind the overwhelming success of this industry. However, purification of proteins is not an easy undertaking due to their intricate nature while presently employed procedures for this purpose, regrettably, are both costly, and labor- and time-intensive in addition to being unsettling on proteins structural conformity. ATPS has accumulated a lot of interest from the scientific community due to its mild operating conditions, high recovery yield, ease of scaling it up, and its cost-effective and environment friendly nature. This review tries to amass some accounts concerning the utility of ATPS for the separation and purification of proteins. Some auspicious clues in this regard can be witnessed along with a few loopholes which need to be addressed before this technique can truly demonstrate its potential vis-à-vis industrial protein purification. Overall, a polymer – salt (citrates in particular) ATPS with an added inert supplementary salt can be regarded as a better option for purifying proteins.

中文翻译：

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ATPS：“水两相系统”作为蛋白质加工食品行业的“蛋白质分离解决方案”

每个人都需要食物以提供营养价值和风味，而一个国家的经济增长取决于蓬勃发展的盈利产业。食品工业有效地满足了这两种需求。可以将蛋白质正确地视为该行业取得巨大成功的推动力。然而，由于蛋白质的复杂性质，纯化蛋白质不是一件容易的事，令人遗憾的是，目前为此目的所采用的方法除了使蛋白质结构顺应性不稳定之外，既昂贵又费时费力。由于ATPS的操作条件温和，回收率高，易于扩展，具有成本效益和环境友好的性质，因此引起了科学界的广泛关注。这篇综述试图积累一些有关ATPS在分离和纯化蛋白质方面的实用性的报道。在这方面，可以看到一些吉祥的线索以及一些漏洞，在此技术真正发挥其潜力之前，需要解决这些漏洞。相对于工业蛋白质纯化。总体而言，添加了惰性补充盐的聚合物-盐（尤其是柠檬酸盐）ATPS可以被视为纯化蛋白质的更好选择。