In this work, the uncertainty estimation for the determination of ametryn, carbofuran, atrazine, carbaryl, and methyl parathion in papaya and avocado is presented, along with other validation parameters. The analytical method was developed using Quick, Easy, Cheap, Effective, Rugged, and Safe extraction and ultrahigh performance liquid chromatography coupled to a triple quadrupole mass detector. The method validation showed that the linear correlation coefficients were higher than 0.99 for both fruits. The limits of detection for avocado and papaya were in the range of 0.022 to 0.46 and 0.003 to 0.109 μg/g, respectively. Intermediate precision varied from 5.3% to 13.0% in papaya, and from 4.8% to 20.2% in avocado. Recoveries obtained for each pesticide in both matrices ranged between 61.3% and 119.0%. Matrix effect was calculated for all compounds in both fruits. Finally, the overall uncertainty was lower than 36% for both fruits. PRACTICAL APPLICATION The present analytical method could be used for pesticides determination in different kind of fruits as papaya and avocado and as a practical guide for uncertainty and matrix effect determination.

中文翻译：

---

三重四极杆质谱联用超高效液相色谱分析鳄梨和木瓜中农药的方法：验证和不确定性评估

在这项工作中，介绍了测定木瓜和鳄梨中苎英、克百威、阿特拉津、西维因和甲基对硫磷的不确定度估计，以及其他验证参数。该分析方法是使用快速、简单、廉价、有效、耐用和安全的萃取以及与三重四极杆质量检测器耦合的超高效液相色谱法开发的。方法验证表明，两种水果的线性相关系数均高于 0.99。鳄梨和木瓜的检测限分别在 0.022 到 0.46 和 0.003 到 0.109 μg/g 的范围内。木瓜的中间精度从 5.3% 到 13.0% 不等，鳄梨的中间精度从 4.8% 到 20.2% 不等。两种基质中每种农药的回收率介于 61.3% 和 119.0% 之间。计算两种水果中所有化合物的基质效应。最后，两种水果的总体不确定性均低于 36%。实际应用 本分析方法可用于木瓜和鳄梨等不同水果中农药的测定，并可作为不确定度和基质效应测定的实用指南。